Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Immunohistochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- 11871-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SH2D1B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.37 mg/mL
- Storage
- -20°C
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- HL-60 cells were subjected to SDS PAGE followed by western blot with 11871-1-AP (SH2D1B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
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- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 11871-1-AP (SH2D1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
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- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 11871-1-AP (SH2D1B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
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- Experimental details
- Raji cells were subjected to SDS PAGE followed by western blot with 11871-1-AP (SH2D1B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
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- Experimental details
- Mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11871-1-AP (SH2D1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney tissue slide using 11871-1-AP ( SH2D1B Antibody) at dilution of 1:200 (under 10x lens).
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney tissue slide using 11871-1-AP ( SH2D1B Antibody) at dilution of 1:200 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- 1X10^6 Jurkat cells were stained with 0.2ug SH2D1B antibody (11871-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.