Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-31533 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NUF2 Monoclonal Antibody (GT644)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Keep as concentrated solution. Positive Control: HeLa, HeLa NE. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- GT644
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using NUF2 Monoclonal Antibody (GT644) (Product # MA5-31533). HeLa whole cell and nuclear extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with NUF2 Monoclonal Antibody (GT644) (Product # MA5-31533) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NUF2 in HeLa whole cell and nuclear extracts using NUF2 monoclonal antibody (Product # MA5-31533) using 30 µg of sample at a dilution of 1:1000. Prior to incubation with primary antibody, the sample was separated on 10% SDS-PAGE.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of NUF2 in HeLa whole cell extracts with NUF2 monoclonal antibody (Product # MA5-31533) using 5 µg of sample. Sample was then incubated with anti-mouse IgG secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of NUF2 in HeLa whole cell extracts with NUF2 monoclonal antibody (Product # MA5-31533) using 5 µg of sample. Sample was then incubated with anti-mouse IgG secondary antibody.
