Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [1]
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Validation data
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- Product number
- MA5-34730 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NUF2 Recombinant Rabbit Monoclonal Antibody (JG34-64)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: K562, PC-3M, THP-1, A431.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- JG34-64
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Nuf2 in Positive control: Line 1: K562 Line 2: PC-3M Line 3: THP-1. Samples were incubated with Nuf2 monoclonal antibody (Product # MA5-34730), at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of Nuf2 in A431 cells (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Samples were incubated with Nuf2 monoclonal antibody (Product # MA5-34730) at a dilution of 1:50, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.