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Western blot
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- Antibody Data
- Antigen structure
- References [18]
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- Validations
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- AF-233-NA - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal FGF basic/FGF2/bFGF Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human and bovine FGF basic/FGF2/bFGF in direct ELISAs and Western blots. In direct ELISAs, approximately 75% cross-reactivity with recombinant mouse FGF basic/FGF2/bFGF is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Metastatic Brain Tumors Disrupt the Blood-Brain Barrier and Alter Lipid Metabolism by Inhibiting Expression of the Endothelial Cell Fatty Acid Transporter Mfsd2a.
Multicolor quantitative confocal imaging cytometry.
Tumor-associated B-cells induce tumor heterogeneity and therapy resistance.
Pericytes impair capillary blood flow and motor function after chronic spinal cord injury.
Peroxynitrite upregulates angiogenic factors VEGF-A, BFGF, and HIF-1α in human corneal limbal epithelial cells.
Endothelial heparan sulfate 6-O-sulfation levels regulate angiogenic responses of endothelial cells to fibroblast growth factor 2 and vascular endothelial growth factor.
Inhibition of PAI-1 induces neutrophil-driven neoangiogenesis and promotes tissue regeneration via production of angiocrine factors in mice.
The fibroblast-derived paracrine factor neuregulin-1 has a novel role in regulating the constitutive color and melanocyte function in human skin.
IFATS collection: Fibroblast growth factor-2-induced hepatocyte growth factor secretion by adipose-derived stromal cells inhibits postinjury fibrogenesis through a c-Jun N-terminal kinase-dependent mechanism.
Temporally expressed PDGF and FGF-2 regulate embryonic coronary artery formation and growth.
A CXCL5- and bFGF-dependent effect of PDGF-B-activated fibroblasts in promoting trafficking and differentiation of bone marrow-derived mesenchymal stem cells.
Pancreatic stellate cells: partners in crime with pancreatic cancer cells.
Agrin, a novel basement membrane component in human and rat liver, accumulates in cirrhosis and hepatocellular carcinoma.
Positive correlation between estradiol and vascular endothelial growth factor but not fibroblast growth factor-2 in normal human breast tissue in vivo.
FGFR2b signaling regulates ex vivo submandibular gland epithelial cell proliferation and branching morphogenesis.
Differential role of platelet granular mediators in angiogenesis.
Proangiogenic properties of human myeloma cells: production of angiopoietin-1 and its potential relationship to myeloma-induced angiogenesis.
The anti-HIV cytokine midkine binds the cell surface-expressed nucleolin as a low affinity receptor.
Tiwary S, Morales JE, Kwiatkowski SC, Lang FF, Rao G, McCarty JH
Scientific reports 2018 May 29;8(1):8267
Scientific reports 2018 May 29;8(1):8267
Multicolor quantitative confocal imaging cytometry.
Coutu DL, Kokkaliaris KD, Kunz L, Schroeder T
Nature methods 2018 Jan;15(1):39-46
Nature methods 2018 Jan;15(1):39-46
Tumor-associated B-cells induce tumor heterogeneity and therapy resistance.
Somasundaram R, Zhang G, Fukunaga-Kalabis M, Perego M, Krepler C, Xu X, Wagner C, Hristova D, Zhang J, Tian T, Wei Z, Liu Q, Garg K, Griss J, Hards R, Maurer M, Hafner C, Mayerhöfer M, Karanikas G, Jalili A, Bauer-Pohl V, Weihsengruber F, Rappersberger K, Koller J, Lang R, Hudgens C, Chen G, Tetzlaff M, Wu L, Frederick DT, Scolyer RA, Long GV, Damle M, Ellingsworth C, Grinman L, Choi H, Gavin BJ, Dunagin M, Raj A, Scholler N, Gross L, Beqiri M, Bennett K, Watson I, Schaider H, Davies MA, Wargo J, Czerniecki BJ, Schuchter L, Herlyn D, Flaherty K, Herlyn M, Wagner SN
Nature communications 2017 Sep 19;8(1):607
Nature communications 2017 Sep 19;8(1):607
Pericytes impair capillary blood flow and motor function after chronic spinal cord injury.
Li Y, Lucas-Osma AM, Black S, Bandet MV, Stephens MJ, Vavrek R, Sanelli L, Fenrich KK, Di Narzo AF, Dracheva S, Winship IR, Fouad K, Bennett DJ
Nature medicine 2017 Jun;23(6):733-741
Nature medicine 2017 Jun;23(6):733-741
Peroxynitrite upregulates angiogenic factors VEGF-A, BFGF, and HIF-1α in human corneal limbal epithelial cells.
Ashki N, Chan AM, Qin Y, Wang W, Kiyohara M, Lin L, Braun J, Wadehra M, Gordon LK
Investigative ophthalmology & visual science 2014 Mar 19;55(3):1637-46
Investigative ophthalmology & visual science 2014 Mar 19;55(3):1637-46
Endothelial heparan sulfate 6-O-sulfation levels regulate angiogenic responses of endothelial cells to fibroblast growth factor 2 and vascular endothelial growth factor.
Ferreras C, Rushton G, Cole CL, Babur M, Telfer BA, van Kuppevelt TH, Gardiner JM, Williams KJ, Jayson GC, Avizienyte E
The Journal of biological chemistry 2012 Oct 19;287(43):36132-46
The Journal of biological chemistry 2012 Oct 19;287(43):36132-46
Inhibition of PAI-1 induces neutrophil-driven neoangiogenesis and promotes tissue regeneration via production of angiocrine factors in mice.
Tashiro Y, Nishida C, Sato-Kusubata K, Ohki-Koizumi M, Ishihara M, Sato A, Gritli I, Komiyama H, Sato Y, Dan T, Miyata T, Okumura K, Tomiki Y, Sakamoto K, Nakauchi H, Heissig B, Hattori K
Blood 2012 Jun 28;119(26):6382-93
Blood 2012 Jun 28;119(26):6382-93
The fibroblast-derived paracrine factor neuregulin-1 has a novel role in regulating the constitutive color and melanocyte function in human skin.
Choi W, Wolber R, Gerwat W, Mann T, Batzer J, Smuda C, Liu H, Kolbe L, Hearing VJ
Journal of cell science 2010 Sep 15;123(Pt 18):3102-11
Journal of cell science 2010 Sep 15;123(Pt 18):3102-11
IFATS collection: Fibroblast growth factor-2-induced hepatocyte growth factor secretion by adipose-derived stromal cells inhibits postinjury fibrogenesis through a c-Jun N-terminal kinase-dependent mechanism.
Suga H, Eto H, Shigeura T, Inoue K, Aoi N, Kato H, Nishimura S, Manabe I, Gonda K, Yoshimura K
Stem cells (Dayton, Ohio) 2009 Jan;27(1):238-49
Stem cells (Dayton, Ohio) 2009 Jan;27(1):238-49
Temporally expressed PDGF and FGF-2 regulate embryonic coronary artery formation and growth.
Tomanek RJ, Hansen HK, Christensen LP
Arteriosclerosis, thrombosis, and vascular biology 2008 Jul;28(7):1237-43
Arteriosclerosis, thrombosis, and vascular biology 2008 Jul;28(7):1237-43
A CXCL5- and bFGF-dependent effect of PDGF-B-activated fibroblasts in promoting trafficking and differentiation of bone marrow-derived mesenchymal stem cells.
Nedeau AE, Bauer RJ, Gallagher K, Chen H, Liu ZJ, Velazquez OC
Experimental cell research 2008 Jul 1;314(11-12):2176-86
Experimental cell research 2008 Jul 1;314(11-12):2176-86
Pancreatic stellate cells: partners in crime with pancreatic cancer cells.
Vonlaufen A, Joshi S, Qu C, Phillips PA, Xu Z, Parker NR, Toi CS, Pirola RC, Wilson JS, Goldstein D, Apte MV
Cancer research 2008 Apr 1;68(7):2085-93
Cancer research 2008 Apr 1;68(7):2085-93
Agrin, a novel basement membrane component in human and rat liver, accumulates in cirrhosis and hepatocellular carcinoma.
Tátrai P, Dudás J, Batmunkh E, Máthé M, Zalatnai A, Schaff Z, Ramadori G, Kovalszky I
Laboratory investigation; a journal of technical methods and pathology 2006 Nov;86(11):1149-60
Laboratory investigation; a journal of technical methods and pathology 2006 Nov;86(11):1149-60
Positive correlation between estradiol and vascular endothelial growth factor but not fibroblast growth factor-2 in normal human breast tissue in vivo.
Dabrosin C
Clinical cancer research : an official journal of the American Association for Cancer Research 2005 Nov 15;11(22):8036-41
Clinical cancer research : an official journal of the American Association for Cancer Research 2005 Nov 15;11(22):8036-41
FGFR2b signaling regulates ex vivo submandibular gland epithelial cell proliferation and branching morphogenesis.
Steinberg Z, Myers C, Heim VM, Lathrop CA, Rebustini IT, Stewart JS, Larsen M, Hoffman MP
Development (Cambridge, England) 2005 Mar;132(6):1223-34
Development (Cambridge, England) 2005 Mar;132(6):1223-34
Differential role of platelet granular mediators in angiogenesis.
Brill A, Elinav H, Varon D
Cardiovascular research 2004 Aug 1;63(2):226-35
Cardiovascular research 2004 Aug 1;63(2):226-35
Proangiogenic properties of human myeloma cells: production of angiopoietin-1 and its potential relationship to myeloma-induced angiogenesis.
Giuliani N, Colla S, Lazzaretti M, Sala R, Roti G, Mancini C, Bonomini S, Lunghi P, Hojden M, Genestreti G, Svaldi M, Coser P, Fattori PP, Sammarelli G, Gazzola GC, Bataille R, Almici C, Caramatti C, Mangoni L, Rizzoli V
Blood 2003 Jul 15;102(2):638-45
Blood 2003 Jul 15;102(2):638-45
The anti-HIV cytokine midkine binds the cell surface-expressed nucleolin as a low affinity receptor.
Said EA, Krust B, Nisole S, Svab J, Briand JP, Hovanessian AG
The Journal of biological chemistry 2002 Oct 4;277(40):37492-502
The Journal of biological chemistry 2002 Oct 4;277(40):37492-502
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- FGF basic in Human Placenta. FGF basic was detected in immersion fixed paraffin-embedded sections of human placenta using Goat Anti-Human FGF basic Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-233-NA) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to trophoblast cells in chorionic villi. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by FGF basic and Neutralization by Human FGF basic Antibody. Bovine FGF basic (Catalog # 133-FB) stimulates proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Bovine FGF basic (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human FGF basic Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-233-NA). The ND50 is typically 0.08-0.4 µg/mL.
