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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- AB-33-NA - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal FGF basic/FGF2/bFGF Antibody
- Antibody type
- Polyclonal
- Description
- Protein A or G purified. Detects bovine FGF basic/FGF2/bFGF in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with FGF acidic and recombinant human (rh) beta-ECGF is observed and less than 1% cross-reactivity with rhFGF-4, rhFGF-5, rhFGF-6, rhFGF-7, recombinant mouse (rm) FGF-8b, rmFGF-8c, rhFGF-9, rhFGF-10, rmFGF-15, rhFGF-17, and rhFGF-18 is observed. Neutralizes the biological activity of bovine FGF basic/FGF2/bFGF and will also neutralize the biological activity of recombinant human FGF basic/FGF2/bFGF.
- Reactivity
- Bovine
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 1 mg
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Maternal obesity and overnutrition alter fetal growth rate and cotyledonary vascularity and angiogenic factor expression in the ewe.
Mediation of electronegative low-density lipoprotein signaling by LOX-1: a possible mechanism of endothelial apoptosis.
Dedicated epithelial recipient cells determine pigmentation patterns.
Wnt regulation of progenitor maturation in the cortex depends on Shh or fibroblast growth factor 2.
Ma Y, Zhu MJ, Zhang L, Hein SM, Nathanielsz PW, Ford SP
American journal of physiology. Regulatory, integrative and comparative physiology 2010 Jul;299(1):R249-58
American journal of physiology. Regulatory, integrative and comparative physiology 2010 Jul;299(1):R249-58
Mediation of electronegative low-density lipoprotein signaling by LOX-1: a possible mechanism of endothelial apoptosis.
Lu J, Yang JH, Burns AR, Chen HH, Tang D, Walterscheid JP, Suzuki S, Yang CY, Sawamura T, Chen CH
Circulation research 2009 Mar 13;104(5):619-27
Circulation research 2009 Mar 13;104(5):619-27
Dedicated epithelial recipient cells determine pigmentation patterns.
Weiner L, Han R, Scicchitano BM, Li J, Hasegawa K, Grossi M, Lee D, Brissette JL
Cell 2007 Sep 7;130(5):932-42
Cell 2007 Sep 7;130(5):932-42
Wnt regulation of progenitor maturation in the cortex depends on Shh or fibroblast growth factor 2.
Viti J, Gulacsi A, Lillien L
The Journal of neuroscience : the official journal of the Society for Neuroscience 2003 Jul 2;23(13):5919-27
The Journal of neuroscience : the official journal of the Society for Neuroscience 2003 Jul 2;23(13):5919-27
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by FGF basic and Neutralization by Bovine FGF basic Antibody. Bovine FGF basic (Catalog # 133-FB) stimulates proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Bovine FGF basic (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-Bovine FGF basic Polyclonal Antibody (Catalog # AB-33-NA). The ND50 is typically 1.5-4.5 µg/mL.
