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Western blot
Functional assayAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- ELISA [1]
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- Product number
- DP3506S - Provider product page
- Provider
- OriGene
- Product name
- FGF2 rabbit polyclonal antibody, Azide Free
- Antibody type
- Polyclonal
- Description
- FGF2 rabbit polyclonal antibody, Azide Free
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- FGF2
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- Lyophilized
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western analysis of recombinant human (DA3503XC), mouse and rat FGF-2 (basic) using a polyclonal antibody directed against recombinant human FGF-2. As expected the antibody cross reacts with the mouse and rat proteins.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Test of cross-reactivity of anti-Human FGF-2 polyclonal antibody by Western blot analysis. Recombinant human, mouse and Rat FGF-2 produced in E. coli were loaded under reducing conditions in 15% SDS-polyacrylamide gel. Anti-human FGF-2 recognizes also the mouse and the rat form.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- FGF-2 Sandwich-ELISA using a polyclonal Goat anti-human FGF-2 as capture antibody and recombinant human FGF-2 ( DA3503X) as standard. The polyclonal rabbit anti-human FGF-2 antibody ( DP3506)in combination with a goat anti-rabbit-Biotin was used for detection.
- Validation comment
- ELISA
