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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- TA500012 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500012, RRID:AB_2104314
- Product name
- bFGF (FGF2) mouse monoclonal antibody, clone OTI3D9 (formerly 3D9)
- Antibody type
- Monoclonal
- Description
- bFGF (FGF2) mouse monoclonal antibody, clone OTI3D9 (formerly 3D9)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- FGF2
- Isotype
- IgG
- Antibody clone number
- OTI3D9
- Vial size
- 100 µl
- Concentration
- NULL
Submitted references Using magnetic nanoparticles for gene transfer to neural stem cells: stem cell propagation method influences outcomes.
Pickard MR, Adams CF, Barraud P, Chari DM
Journal of functional biomaterials 2015 Apr 24;6(2):259-76
Journal of functional biomaterials 2015 Apr 24;6(2):259-76
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BFGF (RC217426, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BFGF.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 4 different cell lines by using anti-BFGF monoclonal antibody at 1:200 dilution.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-BFGF mouse monoclonal antibody (TA500012) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BFGF(RC217426).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-BFGF mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500012)
- Validation comment
- IHC
