MA5-15276

antibody from Invitrogen Antibodies

Targeting: FGF2 FGFB

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

MA5-15276

Provider

Invitrogen Antibodies

Product name

FGF2 Monoclonal Antibody (2H5G2C11)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-15276 targets FGF2 in indirect ELISA, IHC and WB applications and shows reactivity with Human samples. The MA5-15276 immunogen is purified recombinant fragment of FGF2 expressed in E. Coli.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

2H5G2C11

Vial size

100 µL

Concentration

Conc. Not Determined

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

c-Myc-mediated repression of miR-15-16 in hypoxia is induced by increased HIF-2α and promotes tumor angiogenesis and metastasis by upregulating FGF2.

Xue G, Yan HL, Zhang Y, Hao LQ, Zhu XT, Mei Q, Sun SH
Oncogene 2015 Mar 12;34(11):1393-406

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of FGF2 was performed using 80% confluent log phase BJ and T-47D, control and PTI treated, cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with FGF2 Monoclonal Antibody (2H5G2C11) (Product # MA5-15276) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766), (1:2500 dilution), for 45 minutes at room temperature (Panel a,e,i: Green). Nuclei (Panel b,f,j: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c,g,k: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image of untreated BJ cells showing faint staining for FGF2 that is enhanced upon PTI treatment (Panel h). Panel l represents T-47D cells showing no staining for FGF2 upon PTI treatment. The images were captured at 60X magnification. FGF2 follows type I unconventional secretory pathway where it undergoes self-oligomerization and interacts with the inner and outer leaflets of the plasma membrane for its release. The punctate pattern of FGF2 seen in panel d and h is similar to that shown by Steringer et.al. of the intermediates and oligomers of FGF2 for membrane insertion.