Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- RP1006 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Fibroblast growth factor 2 FGF2 Antibody
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for FGF basic/FGF2 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: ELISA. Reactive species: Human. FGF basic/FGF2 information: Molecular Weight: 30770 MW; Subcellular Localization: Secreted. Nucleus. Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57; Tissue Specificity: Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non- cancerous liver tissue.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
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Supportive validation
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- Boster Biological Technology (provider)
- Main image
- Experimental details
- Anti- FGF2 antibody, RP1006, Western blottingAll lanes: Anti FGF2(RP1006) at 0.5ug/mlLane 1: Recombinant Human FGF2 Protein 10ngLane 2: Recombinant Human FGF2 Protein 5ngLane 3: Recombinant Human FGF2 Protein 2.5ngPredicted bind size: 17KDObserved bind size: 17KD
- Additional image