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- Antibody Data
- Antigen structure
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- Product number
- PA5-50009 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CHPF Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- The antibody detects endogenous levels of total CHPF protein.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Chondroitin polymerizing factor promotes breast carcinoma cell proliferation, invasion and migration and affects expression of epithelial-mesenchymal transition-related markers.
Li Y, Gong H, Feng L, Mao D, Xiao Y, Wang Y, Huang L
FEBS open bio 2021 Feb;11(2):423-434
FEBS open bio 2021 Feb;11(2):423-434
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CHPF in extracts from LOVO cells and K562 cells. Samples were probed using CHPF antibody (Product # PA5-50009).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of CHPF in MCF-7 cells. Samples were probed using a CHPF polyclonal antibody (Product # PA5-50009).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CHPF in paraffin-embedded Human cervix carcinoma tissue using CHPF Polyclonal Antibody (Product # PA5-50009).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 2 Fig. Detection of knockdown and overexpression of CHPF efficiency. qRT-PCR (A, B) and western blotting (C) were used to test mRNA (A, B) and protein (C) levels of CHPF after transfection with si-CHPF#1, si-CHPF#2 and si-con. qRT-PCR (D, E) and western blotting (F) were used to test mRNA (D, E) and protein (F) levels of CHPF after transfection with pcDNA3.1-CHPF and pcDNA3.1. ** P < 0.01 vs. si-con group or vector group. Data are presented as the mean +- SD for three independent experiments. Statistical analyses in (A), (B) and (C) were performed by ANOVA followed by a Bonferroni post-hoc test. Statistical analyses in (D), (E) and (F) were analyzed using Student's t -test.
