A300-663A

antibody from Invitrogen Antibodies

Targeting: TIPRL dJ69E11.3, MGC3794, TIP41, TIPRL1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A300-663A

Provider

Invitrogen Antibodies

Product name

Tip41 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

The recommended shelf life for this product is 1 year from date of receipt.

Concentration

0.20 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human Tip41 by western blot. Samples: Whole cell lysate (50 µg) from Jurkat, HEK293T, A-549, MCF-7, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Tip41 antibody (Product # A300-663A lot 2) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of mouse Tip41 by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Tip41 antibody (Product # A300-663A lot 2) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 75 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human and mouse Tip41 by western blot (h & m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP; 20% of IP loaded), 293T (T; 50 µg) and mouse NIH3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-Tip41 antibody BL3057 (Cat. No. A300-663A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. Efficiency of immunoprecipitation of Tip41 was much less when using rabbit anti-Tip41 antibody BL3056 (Cat. No. A300-662A) or BL3058. Detection: Chemiluminescence with exposure times of 3 seconds (A and B).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human Tip41 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- Tip41 (Product # A300-663A; lot 2) used at a dilution of 1:200 (1 µg/mL). Detection: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human Tip41 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- Tip41 (Cat. No. A300-663A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: DAB.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human Tip41 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Tip41 antibody (Product # A300-663A lot 2) used for IP at 6 µg per reaction. Tip41 was also immunoprecipitated by a previous lot of this antibody (A300-663A lot 1). For blotting immunoprecipitated Tip41 (Product # A300-663A) was used at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.