CF500853

antibody from Invitrogen Antibodies

Targeting: TIPRL dJ69E11.3, MGC3794, TIP41, TIPRL1

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

CF500853

Provider

Invitrogen Antibodies

Product name

TIPRL Monoclonal Antibody (OTI3D11), TrueMAB™

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

OTI3D11

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TIPRL (RC223912, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-TIPRL. Positive lysates LY403496 (100 µg) and LC403496 (20 µg) can be purchased separately from OriGene.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of Hela cells, using anti-TIPRL antibody (TA500853), (Red) compared to a nonspecific negative control antibody (TA50011)(Blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of Jurkat cells, using anti-TIPRL antibody (TA500853), (Red) compared to a nonspecific negative control antibody (TA50011)(Blue).