Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- F54444 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- EphA4 Antibody
- Antibody type
- Polyclonal
- Description
- SAS precipitation
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 0.08 µl, 0.4 µl
- Storage
- Aliquot the EphA4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) HeLa and 2) NCI-H460 cell lysate with EphA4 antibody. Expected molecular weight: 110-130 kDa depending on glycosylation level.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with EphA4 antibody.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human breast cancer tissue with EphA4 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human CCRF-CEM cells with EphA4 antibody; Blue=isotype control, Green= EphA4 antibody.