Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [3]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- PA5-14578 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EphA4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with chicken, mouse and Xenopus based on sequence homology.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 1.38 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using an EphA4 polyclonal antibody (Product # PA5-14578) in HeLa cell lysate. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of EphA4 (arrow) using an EphA4 polyclonal antibody (Product # PA5-14578) in 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the EphA4 gene (Lane 2).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Methanol/Acetone fixed human stem cell using an EphA4 polyclonal antibody (Product # PA5-14578) to detect Eph4A (blue) and endothelial Lectin (red).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Methanol/Acetone fixed human stem cell using an EphA4 polyclonal antibody (Product # PA5-14578) to detect Eph4A (blue) and endothelial Lectin (red).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Methanol/Acetone fixed human stem cell using an EphA4 polyclonal antibody (Product # PA5-14578) to detect Eph4A (blue) and endothelial Lectin (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of formalin-fixed, paraffin-embedded human cancer tissue using an EphA4 polyclonal antibody (Product # PA5-14578), followed by HRP-conjugated secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of CEM cells using an EphA4 polyclonal antibody (Product # PA5-14578) (bottom), compared to a negative control cell (top) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody