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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [3]
- Immunohistochemistry [1]
- Flow cytometry [2]
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- Product number
- PA5-14578 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EphA4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with chicken, mouse and Xenopus based on sequence homology.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 μL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Methanol/Acetone fixed human stem cell using an EphA4 polyclonal antibody (Product # PA5-14578) to detect Eph4A (blue) and endothelial Lectin (red).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Methanol/Acetone fixed human stem cell using an EphA4 polyclonal antibody (Product # PA5-14578) to detect Eph4A (blue) and endothelial Lectin (red).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Methanol/Acetone fixed human stem cell using an EphA4 polyclonal antibody (Product # PA5-14578) to detect Eph4A (blue) and endothelial Lectin (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of EphA4 in formalin-fixed and paraffin-embedded human cancer tissue. Samples were incubated with EphA4 polyclonal antibody (Product # PA5-14578) which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of CEM cells using an EphA4 polyclonal antibody (Product # PA5-14578) (bottom), compared to a negative control cell (top) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of EphA4 in CEM cells (bottom histogram). Samples were incubated with EphA4 polyclonal antibody (Product # PA5-14578) followed by FITC-conjugated goat-anti-rabbit secondary antibody. Negative control cell (top histogram).
