PA5-27998

antibody from Invitrogen Antibodies

Targeting: EPHA4 Hek8, TYRO1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-27998

Provider

Invitrogen Antibodies

Product name

EphA4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Recommended positive controls: A549, HeLa, HepG2, HCT116, 3xFlag-human EPHA4-transfected 293T, human brain, mouse brain, rat brain, mouse hippocampus, DDDDk-tagged EPHA4-transfected 293T. Predicted reactivity: Mouse (99%), Rat (99%), Xenopus laevis (85%), Pig (99%), Chicken (93%), Rhesus Monkey (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 μL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Eph receptor A4 antibody detects Eph receptor A4 protein by western blot analysis. Human tissue extracts (30 µg) was separated by 5 % SDS-PAGE, and the membrane was blotted with Eph receptor A4 antibody EphA4 Polyclonal Antibody (Product # PA5-27998) diluted by 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using EphA4 Polyclonal Antibody (Product # PA5-27998). Sample (30 µg of whole cell lysate). Lane A: HCT116 . 7.5% SDS PAGE. EphA4 Polyclonal Antibody (Product # PA5-27998) diluted at 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of EphA4 was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a EphA4 Polyclonal Antibody (Product # PA5-27998) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of EphA4 was performed by separating 50 µg of Various tissue extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a EphA4 Polyclonal Antibody (Product # PA5-27998) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of EphA4 was performed by separating 50 µg of Mouse tissue extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a EphA4 Polyclonal Antibody (Product # PA5-27998) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-EphA4 Polyclonal Antibody (Product # PA5-27998) and ~110 kDa band corresponding to EphA4 was observed across the high expressing models (Mouse brain and Rat brain) and was absent in low expressing models (Mouse cerebellum, Mouse liver and Mouse pancreas). An uncharacterized band (*) at ~40 kDa was also observed. Tissue extracts (30 µg lysate) of Mouse brain (Lane 1), Mouse cerebellum (Lane 2), Rat brain (Lane 3), Mouse liver (Lane 4) and Mouse pancreas (Lane 5) were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).