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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PA5-42767 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SGPP2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide sequence: SKPAESLPVI QNIPPLTTYM LVLGLTKFAV GIVLILLVRQ LVQNLSLQVL
- Concentration
- 1 mg/mL
Submitted references Sphingolipid Pathway as a Source of Vulnerability in IDH1(mut) Glioma.
Dowdy T, Zhang L, Celiku O, Movva S, Lita A, Ruiz-Rodado V, Gilbert MR, Larion M
Cancers 2020 Oct 10;12(10)
Cancers 2020 Oct 10;12(10)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human HepG2 cell lysate using an anti-SGPP2 polyclonal antibody (Product # PA5-42767).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts of MKN45 (Lane 1), SK-OV-3 (Lane 2), A-431 (Lane 3), THP-1 (Lane 4), COS-7 (Lane 5) and A549 (Lane 6). The blot was probed with Anti-SGPP2 Polyclonal Antibody (Product # PA5-42767, 2 µg/ml) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 35 kDa band corresponding to SGPP2 was observed across the cell lines tested.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of human kidney tissue using an anti-SGPP2 polyclonal antibody (Product # PA5-42767).
