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- Antibody Data
- Antigen structure
- References [19]
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- Immunohistochemistry [1]
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- Product number
- PB9099 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Collagen IV/COL4A1 Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for COLLAGEN IV/COL4A1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB, IHC-P, IF. Reactive species: Human. COLLAGEN IV/COL4A1 information: Molecular Weight: 138564 MW; Subcellular Localization: Secreted, extracellular space, extracellular matrix ; Tissue Specificity: Highly expressed in placenta.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references The Role of Leukotriene B(4) in Cow Metritis.
Ibrolipim attenuates early-stage nephropathy in diet-induced diabetic minipigs: Focus on oxidative stress and fibrogenesis.
Cardioprotection of Sheng Mai Yin a classic formula on adriamycin induced myocardial injury in Wistar rats.
Neutral endopeptidase and natriuretic peptide receptors participate in the regulation of C-type natriuretic peptide expression in renal interstitial fibrosis.
Sesamin Ameliorates High-Fat Diet-Induced Dyslipidemia and Kidney Injury by Reducing Oxidative Stress.
Protective effects of Danggui Buxue Tang on renal function, renal glomerular mesangium and heparanase expression in rats with streptozotocin-induced diabetes mellitus.
Engineering blood vessels through micropatterned co-culture of vascular endothelial and smooth muscle cells on bilayered electrospun fibrous mats with pDNA inoculation.
Effects of uPA on mesangial matrix changes in the kidney of diabetic rats.
Electrosprayed microparticles with loaded pDNA-calcium phosphate nanoparticles to promote the regeneration of mature blood vessels.
Transcriptome sequencing reveals differences between primary and secondary hair follicle-derived dermal papilla cells of the Cashmere goat (Capra hircus).
Resveratrol attenuates diabetic nephropathy via modulating angiogenesis.
Renoprotective effects of berberine and its possible molecular mechanisms in combination of high-fat diet and low-dose streptozotocin-induced diabetic rats.
Overexpressed C-type natriuretic peptide serves as an early compensatory response to counteract extracellular matrix remodeling in unilateral ureteral obstruction rats.
Effects of aminoguanidine and vitamin C on collagen type IV in diabetic nephropathy rats.
The effect of down-regulation of Smad3 by RNAi on hepatic stellate cells and a carbon tetrachloride-induced rat model of hepatic fibrosis.
Severe insulin resistance and moderate glomerulosclerosis in a minipig model induced by high-fat/ high-sucrose/ high-cholesterol diet.
Rosiglitazone prevents advanced glycation end products-induced renal toxicity likely through suppression of plasminogen activator inhibitor-1.
Protective effects of blocking renin-angiotensin system on the progression of renal injury in glomerulosclerosis.
Advanced glycation end-products induce connective tissue growth factor-mediated renal fibrosis predominantly through transforming growth factor beta-independent pathway.
Wang GQ, Zheng HY, Hou JL, Wang C, Sun HL, Wang L
Journal of veterinary research 2023 Mar;67(1):99-104
Journal of veterinary research 2023 Mar;67(1):99-104
Ibrolipim attenuates early-stage nephropathy in diet-induced diabetic minipigs: Focus on oxidative stress and fibrogenesis.
Liu Y, Li H, Wang S, Yin W, Wang Z
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2020 Sep;129:110321
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2020 Sep;129:110321
Cardioprotection of Sheng Mai Yin a classic formula on adriamycin induced myocardial injury in Wistar rats.
Zhang K, Zhang J, Wang X, Wang L, Pugliese M, Passantino A, Li J
Phytomedicine : international journal of phytotherapy and phytopharmacology 2018 Jan 1;38:1-11
Phytomedicine : international journal of phytotherapy and phytopharmacology 2018 Jan 1;38:1-11
Neutral endopeptidase and natriuretic peptide receptors participate in the regulation of C-type natriuretic peptide expression in renal interstitial fibrosis.
Hu P, Xia X, Xuan Q, Huang BY, Liu SY, Zhang DD, Jiang GM, Xu Y, Qin YH
Journal of receptor and signal transduction research 2017 Feb;37(1):71-83
Journal of receptor and signal transduction research 2017 Feb;37(1):71-83
Sesamin Ameliorates High-Fat Diet-Induced Dyslipidemia and Kidney Injury by Reducing Oxidative Stress.
Zhang R, Yu Y, Deng J, Zhang C, Zhang J, Cheng Y, Luo X, Han B, Yang H
Nutrients 2016 May 9;8(5)
Nutrients 2016 May 9;8(5)
Protective effects of Danggui Buxue Tang on renal function, renal glomerular mesangium and heparanase expression in rats with streptozotocin-induced diabetes mellitus.
Ye TS, Zhang YW, Zhang XM
Experimental and therapeutic medicine 2016 Jun;11(6):2477-2483
Experimental and therapeutic medicine 2016 Jun;11(6):2477-2483
Engineering blood vessels through micropatterned co-culture of vascular endothelial and smooth muscle cells on bilayered electrospun fibrous mats with pDNA inoculation.
Liu Y, Lu J, Li H, Wei J, Li X
Acta biomaterialia 2015 Jan;11:114-25
Acta biomaterialia 2015 Jan;11:114-25
Effects of uPA on mesangial matrix changes in the kidney of diabetic rats.
Cheng H, Chen C, Wang S
Renal failure 2014 Sep;36(8):1322-7
Renal failure 2014 Sep;36(8):1322-7
Electrosprayed microparticles with loaded pDNA-calcium phosphate nanoparticles to promote the regeneration of mature blood vessels.
Guo X, Xia T, Wang H, Chen F, Cheng R, Luo X, Li X
Pharmaceutical research 2014 Apr;31(4):874-86
Pharmaceutical research 2014 Apr;31(4):874-86
Transcriptome sequencing reveals differences between primary and secondary hair follicle-derived dermal papilla cells of the Cashmere goat (Capra hircus).
Zhu B, Xu T, Yuan J, Guo X, Liu D
PloS one 2013;8(9):e76282
PloS one 2013;8(9):e76282
Resveratrol attenuates diabetic nephropathy via modulating angiogenesis.
Wen D, Huang X, Zhang M, Zhang L, Chen J, Gu Y, Hao CM
PloS one 2013;8(12):e82336
PloS one 2013;8(12):e82336
Renoprotective effects of berberine and its possible molecular mechanisms in combination of high-fat diet and low-dose streptozotocin-induced diabetic rats.
Wang FL, Tang LQ, Yang F, Zhu LN, Cai M, Wei W
Molecular biology reports 2013 Mar;40(3):2405-18
Molecular biology reports 2013 Mar;40(3):2405-18
Overexpressed C-type natriuretic peptide serves as an early compensatory response to counteract extracellular matrix remodeling in unilateral ureteral obstruction rats.
Hu P, Wang J, Zhao XQ, Hu B, Lu L, Qin YH
Molecular biology reports 2013 Feb;40(2):1429-41
Molecular biology reports 2013 Feb;40(2):1429-41
Effects of aminoguanidine and vitamin C on collagen type IV in diabetic nephropathy rats.
Li Q, Ao X, Du Y, Li Y, Ou Y, Gong R, Sun X, Yang YX, Wen G
Endocrine 2011 Jun;39(3):251-8
Endocrine 2011 Jun;39(3):251-8
The effect of down-regulation of Smad3 by RNAi on hepatic stellate cells and a carbon tetrachloride-induced rat model of hepatic fibrosis.
Wang ZR, Wang JH, Hu CL, Cao WG, Shen XJ, Wu MY, Shen L, Wu SL
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas 2011 Feb;44(2):91-9
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas 2011 Feb;44(2):91-9
Severe insulin resistance and moderate glomerulosclerosis in a minipig model induced by high-fat/ high-sucrose/ high-cholesterol diet.
Liu Y, Wang Z, Yin W, Li Q, Cai M, Zhang C, Xiao J, Hou H, Li H, Zu X
Experimental animals 2007 Jan;56(1):11-20
Experimental animals 2007 Jan;56(1):11-20
Rosiglitazone prevents advanced glycation end products-induced renal toxicity likely through suppression of plasminogen activator inhibitor-1.
Yu X, Li C, Li X, Cai L
Toxicological sciences : an official journal of the Society of Toxicology 2007 Apr;96(2):346-56
Toxicological sciences : an official journal of the Society of Toxicology 2007 Apr;96(2):346-56
Protective effects of blocking renin-angiotensin system on the progression of renal injury in glomerulosclerosis.
Ji Z, Huang C, Liang C, Chen B, Chen S, Sun W
Cellular & molecular immunology 2005 Apr;2(2):150-4
Cellular & molecular immunology 2005 Apr;2(2):150-4
Advanced glycation end-products induce connective tissue growth factor-mediated renal fibrosis predominantly through transforming growth factor beta-independent pathway.
Zhou G, Li C, Cai L
The American journal of pathology 2004 Dec;165(6):2033-43
The American journal of pathology 2004 Dec;165(6):2033-43
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- IHC analysis of Collagen ??using anti-Collagen ??antibody (PB9099). Collagen ??was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Collagen ??Antibody (PB9099) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
- Additional image
