Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- M01411 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Collagen IV COL4A1 Antibody Picoband™ (monoclonal, 3G3)
- Antibody type
- Monoclonal
- Description
- Mouse IgG monoclonal antibody for Collagen IV detection. Tested with WB, IHC-P, IF in Human.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 3G3
- Vial size
- 100μg/vial
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references The Role of Leukotriene B(4) in Cow Metritis.
Synthetic E-selectin prevents postoperative vascular restenosis by inhibiting nuclear factor κB in rats.
Adenovirus-mediated decorin gene transfection has therapeutic effects in a streptozocin-induced diabetic rat model.
Wang GQ, Zheng HY, Hou JL, Wang C, Sun HL, Wang L
Journal of veterinary research 2023 Mar;67(1):99-104
Journal of veterinary research 2023 Mar;67(1):99-104
Synthetic E-selectin prevents postoperative vascular restenosis by inhibiting nuclear factor κB in rats.
Liu J, Liu Z, Hu X, Zhang Y, Zhang S
Molecular medicine reports 2018 Apr;17(4):5065-5073
Molecular medicine reports 2018 Apr;17(4):5065-5073
Adenovirus-mediated decorin gene transfection has therapeutic effects in a streptozocin-induced diabetic rat model.
Zhang Z, Wu F, Zheng F, Li H
Nephron. Experimental nephrology 2010;116(1):e11-21
Nephron. Experimental nephrology 2010;116(1):e11-21
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- IHC analysis of Collagen IV using anti-Collagen IV antibody (M01411). Collagen IV was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Collagen IV Antibody (M01411) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
- Additional image