Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [34]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 16394-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#16394-1-AP, RRID:AB_2146062
- Product name
- MRPL44 antibody
- Antibody type
- Polyclonal
- Description
- MRPL44 antibody (Cat. #16394-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IHC, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Deficiency of the mitochondrial ribosomal subunit, MRPL50, causes autosomal recessive syndromic premature ovarian insufficiency.
Multi-omics identifies large mitoribosomal subunit instability caused by pathogenic MRPL39 variants as a cause of pediatric onset mitochondrial disease.
Mitochondrial Protein SLIRP Affects Biosynthesis of Cytochrome c Oxidase Subunits in HEK293T Cells.
Pentatricopeptide Protein PTCD2 Regulates COIII Translation in Mitochondria of the HeLa Cell Line.
Serine palmitoyltransferase assembles at ER-mitochondria contact sites.
Non-canonical phosphoglycerate dehydrogenase activity promotes liver cancer growth via mitochondrial translation and respiratory metabolism.
Linear Density Sucrose Gradients to Study Mitoribosomal Biogenesis in Tissue-Specific Knockout Mice.
Integrative Multi-omics Landscape of Non-structural Protein 3 of Severe Acute Respiratory Syndrome Coronaviruses.
An in vitro system to silence mitochondrial gene expression.
Uniparental isodisomy of chromosome 2 causing MRPL44-related multisystem mitochondrial disease.
Mitochondrial mistranslation modulated by metabolic stress causes cardiovascular disease and reduced lifespan.
A deafness-associated tRNA mutation caused pleiotropic effects on the m1G37 modification, processing, stability and aminoacylation of tRNAIle and mitochondrial translation.
MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control.
Protein Stability Buffers the Cost of Translation Attenuation following eIF2α Phosphorylation.
Cardiolipin is required for membrane docking of mitochondrial ribosomes and protein synthesis.
Mutations in the MRPS28 gene encoding the small mitoribosomal subunit protein bS1m in a patient with intrauterine growth retardation, craniofacial dysmorphism and multisystemic involvement.
A variant in MRPS14 (uS14m) causes perinatal hypertrophic cardiomyopathy with neonatal lactic acidosis, growth retardation, dysmorphic features and neurological involvement.
Fidelity of translation initiation is required for coordinated respiratory complex assembly.
Stress signaling and cellular proliferation reverse the effects of mitochondrial mistranslation.
The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis.
Concerted regulation of mitochondrial and nuclear non-coding RNAs by a dual-targeted RNase Z.
Stomatin-like protein 2 deficiency results in impaired mitochondrial translation.
Transcriptomic and proteomic landscape of mitochondrial dysfunction reveals secondary coenzyme Q deficiency in mammals.
A pseudouridine synthase module is essential for mitochondrial protein synthesis and cell viability.
Adult-onset obesity is triggered by impaired mitochondrial gene expression.
Loss of the RNA-binding protein TACO1 causes late-onset mitochondrial dysfunction in mice.
Hierarchical RNA Processing Is Required for Mitochondrial Ribosome Assembly.
A Role for the Mitochondrial Protein Mrpl44 in Maintaining OXPHOS Capacity.
Quality control of mitochondrial protein synthesis is required for membrane integrity and cell fitness.
Mutation in MRPS34 compromises protein synthesis and causes mitochondrial dysfunction.
Mitochondrial RNA Granules Are Centers for Posttranscriptional RNA Processing and Ribosome Biogenesis.
A bifunctional protein regulates mitochondrial protein synthesis.
Whole-exome sequencing identifies a mutation in the mitochondrial ribosome protein MRPL44 to underlie mitochondrial infantile cardiomyopathy.
The mitochondrial RNA-binding protein GRSF1 localizes to RNA granules and is required for posttranscriptional mitochondrial gene expression.
Bakhshalizadeh S, Hock DH, Siddall NA, Kline BL, Sreenivasan R, Bell KM, Casagranda F, Kamalanathan S, Sahoo J, Narayanan N, Naik D, Suryadevara V, Compton AG, Amarasekera SSC, Kapoor R, Jaillard S, Simpson A, Robevska G, van den Bergen J, Pachernegg S, Ayers KL, Thorburn DR, Stroud DA, Hime GR, Sinclair AH, Tucker EJ
Human genetics 2023 Jul;142(7):879-907
Human genetics 2023 Jul;142(7):879-907
Multi-omics identifies large mitoribosomal subunit instability caused by pathogenic MRPL39 variants as a cause of pediatric onset mitochondrial disease.
Amarasekera SSC, Hock DH, Lake NJ, Calvo SE, Grønborg SW, Krzesinski EI, Amor DJ, Fahey MC, Simons C, Wibrand F, Mootha VK, Lek M, Lunke S, Stark Z, Østergaard E, Christodoulou J, Thorburn DR, Stroud DA, Compton AG
Human molecular genetics 2023 Jul 20;32(15):2441-2454
Human molecular genetics 2023 Jul 20;32(15):2441-2454
Mitochondrial Protein SLIRP Affects Biosynthesis of Cytochrome c Oxidase Subunits in HEK293T Cells.
Baleva MV, Piunova U, Chicherin I, Vasilev R, Levitskii S, Kamenski P
International journal of molecular sciences 2023 Dec 20;25(1)
International journal of molecular sciences 2023 Dec 20;25(1)
Pentatricopeptide Protein PTCD2 Regulates COIII Translation in Mitochondria of the HeLa Cell Line.
Baleva MV, Chicherin I, Piunova U, Zgoda V, Patrushev MV, Levitskii S, Kamenski P
International journal of molecular sciences 2022 Nov 17;23(22)
International journal of molecular sciences 2022 Nov 17;23(22)
Serine palmitoyltransferase assembles at ER-mitochondria contact sites.
Aaltonen MJ, Alecu I, König T, Bennett SA, Shoubridge EA
Life science alliance 2022 Feb;5(2)
Life science alliance 2022 Feb;5(2)
Non-canonical phosphoglycerate dehydrogenase activity promotes liver cancer growth via mitochondrial translation and respiratory metabolism.
Shu Y, Hao Y, Feng J, Liu H, Li ST, Feng J, Jiang Z, Ye L, Zhou Y, Sun Y, Zhou Z, Wei H, Gao P, Zhang H, Sun L
The EMBO journal 2022 Dec 1;41(23):e111550
The EMBO journal 2022 Dec 1;41(23):e111550
Linear Density Sucrose Gradients to Study Mitoribosomal Biogenesis in Tissue-Specific Knockout Mice.
Ruzzenente B, Metodiev MD
Methods in molecular biology (Clifton, N.J.) 2021;2224:47-60
Methods in molecular biology (Clifton, N.J.) 2021;2224:47-60
Integrative Multi-omics Landscape of Non-structural Protein 3 of Severe Acute Respiratory Syndrome Coronaviruses.
Shi R, Feng Z, Zhang X
Genomics, proteomics & bioinformatics 2021 Oct;19(5):707-726
Genomics, proteomics & bioinformatics 2021 Oct;19(5):707-726
An in vitro system to silence mitochondrial gene expression.
Cruz-Zaragoza LD, Dennerlein S, Linden A, Yousefi R, Lavdovskaia E, Aich A, Falk RR, Gomkale R, Schöndorf T, Bohnsack MT, Richter-Dennerlein R, Urlaub H, Rehling P
Cell 2021 Nov 11;184(23):5824-5837.e15
Cell 2021 Nov 11;184(23):5824-5837.e15
Uniparental isodisomy of chromosome 2 causing MRPL44-related multisystem mitochondrial disease.
Horga A, Manole A, Mitchell AL, Bugiardini E, Hargreaves IP, Mowafi W, Bettencourt C, Blakely EL, He L, Polke JM, Woodward CE, Dalla Rosa I, Shah S, Pittman AM, Quinlivan R, Reilly MM, Taylor RW, Holt IJ, Hanna MG, Pitceathly RDS, Spinazzola A, Houlden H
Molecular biology reports 2021 Mar;48(3):2093-2104
Molecular biology reports 2021 Mar;48(3):2093-2104
Mitochondrial mistranslation modulated by metabolic stress causes cardiovascular disease and reduced lifespan.
Richman TR, Ermer JA, Siira SJ, Kuznetsova I, Brosnan CA, Rossetti G, Baker J, Perks KL, Cserne Szappanos H, Viola HM, Gray N, Larance M, Hool LC, Zuryn S, Rackham O, Filipovska A
Aging cell 2021 Jul;20(7):e13408
Aging cell 2021 Jul;20(7):e13408
A deafness-associated tRNA mutation caused pleiotropic effects on the m1G37 modification, processing, stability and aminoacylation of tRNAIle and mitochondrial translation.
Meng F, Zhou M, Xiao Y, Mao X, Zheng J, Lin J, Lin T, Ye Z, Cang X, Fu Y, Wang M, Guan MX
Nucleic acids research 2021 Jan 25;49(2):1075-1093
Nucleic acids research 2021 Jan 25;49(2):1075-1093
MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control.
König T, Nolte H, Aaltonen MJ, Tatsuta T, Krols M, Stroh T, Langer T, McBride HM
Nature cell biology 2021 Dec;23(12):1271-1286
Nature cell biology 2021 Dec;23(12):1271-1286
Protein Stability Buffers the Cost of Translation Attenuation following eIF2α Phosphorylation.
Schneider K, Nelson GM, Watson JL, Morf J, Dalglish M, Luh LM, Weber A, Bertolotti A
Cell reports 2020 Sep 15;32(11):108154
Cell reports 2020 Sep 15;32(11):108154
Cardiolipin is required for membrane docking of mitochondrial ribosomes and protein synthesis.
Lee RG, Gao J, Siira SJ, Shearwood AM, Ermer JA, Hofferek V, Mathews JC, Zheng M, Reid GE, Rackham O, Filipovska A
Journal of cell science 2020 Jul 24;133(14)
Journal of cell science 2020 Jul 24;133(14)
Mutations in the MRPS28 gene encoding the small mitoribosomal subunit protein bS1m in a patient with intrauterine growth retardation, craniofacial dysmorphism and multisystemic involvement.
Pulman J, Ruzzenente B, Bianchi L, Rio M, Boddaert N, Munnich A, Rötig A, Metodiev MD
Human molecular genetics 2019 May 1;28(9):1445-1462
Human molecular genetics 2019 May 1;28(9):1445-1462
A variant in MRPS14 (uS14m) causes perinatal hypertrophic cardiomyopathy with neonatal lactic acidosis, growth retardation, dysmorphic features and neurological involvement.
Jackson CB, Huemer M, Bolognini R, Martin F, Szinnai G, Donner BC, Richter U, Battersby BJ, Nuoffer JM, Suomalainen A, Schaller A
Human molecular genetics 2019 Feb 15;28(4):639-649
Human molecular genetics 2019 Feb 15;28(4):639-649
Fidelity of translation initiation is required for coordinated respiratory complex assembly.
Rudler DL, Hughes LA, Perks KL, Richman TR, Kuznetsova I, Ermer JA, Abudulai LN, Shearwood AJ, Viola HM, Hool LC, Siira SJ, Rackham O, Filipovska A
Science advances 2019 Dec;5(12):eaay2118
Science advances 2019 Dec;5(12):eaay2118
Stress signaling and cellular proliferation reverse the effects of mitochondrial mistranslation.
Ferreira N, Perks KL, Rossetti G, Rudler DL, Hughes LA, Ermer JA, Scott LH, Kuznetsova I, Richman TR, Narayana VK, Abudulai LN, Shearwood AJ, Cserne Szappanos H, Tull D, Yeoh GC, Hool LC, Filipovska A, Rackham O
The EMBO journal 2019 Dec 16;38(24):e102155
The EMBO journal 2019 Dec 16;38(24):e102155
The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis.
Lavdovskaia E, Kolander E, Steube E, Mai MM, Urlaub H, Richter-Dennerlein R
Nucleic acids research 2018 Sep 19;46(16):8471-8482
Nucleic acids research 2018 Sep 19;46(16):8471-8482
Concerted regulation of mitochondrial and nuclear non-coding RNAs by a dual-targeted RNase Z.
Siira SJ, Rossetti G, Richman TR, Perks K, Ermer JA, Kuznetsova I, Hughes L, Shearwood AJ, Viola HM, Hool LC, Rackham O, Filipovska A
EMBO reports 2018 Oct;19(10)
EMBO reports 2018 Oct;19(10)
Stomatin-like protein 2 deficiency results in impaired mitochondrial translation.
Mitsopoulos P, Lapohos O, Weraarpachai W, Antonicka H, Chang YH, Madrenas J
PloS one 2017;12(6):e0179967
PloS one 2017;12(6):e0179967
Transcriptomic and proteomic landscape of mitochondrial dysfunction reveals secondary coenzyme Q deficiency in mammals.
Kühl I, Miranda M, Atanassov I, Kuznetsova I, Hinze Y, Mourier A, Filipovska A, Larsson NG
eLife 2017 Nov 14;6
eLife 2017 Nov 14;6
A pseudouridine synthase module is essential for mitochondrial protein synthesis and cell viability.
Antonicka H, Choquet K, Lin ZY, Gingras AC, Kleinman CL, Shoubridge EA
EMBO reports 2017 Jan;18(1):28-38
EMBO reports 2017 Jan;18(1):28-38
Adult-onset obesity is triggered by impaired mitochondrial gene expression.
Perks KL, Ferreira N, Richman TR, Ermer JA, Kuznetsova I, Shearwood AJ, Lee RG, Viola HM, Johnstone VPA, Matthews V, Hool LC, Rackham O, Filipovska A
Science advances 2017 Aug;3(8):e1700677
Science advances 2017 Aug;3(8):e1700677
Loss of the RNA-binding protein TACO1 causes late-onset mitochondrial dysfunction in mice.
Richman TR, Spåhr H, Ermer JA, Davies SM, Viola HM, Bates KA, Papadimitriou J, Hool LC, Rodger J, Larsson NG, Rackham O, Filipovska A
Nature communications 2016 Jun 20;7:11884
Nature communications 2016 Jun 20;7:11884
Hierarchical RNA Processing Is Required for Mitochondrial Ribosome Assembly.
Rackham O, Busch JD, Matic S, Siira SJ, Kuznetsova I, Atanassov I, Ermer JA, Shearwood AM, Richman TR, Stewart JB, Mourier A, Milenkovic D, Larsson NG, Filipovska A
Cell reports 2016 Aug 16;16(7):1874-90
Cell reports 2016 Aug 16;16(7):1874-90
A Role for the Mitochondrial Protein Mrpl44 in Maintaining OXPHOS Capacity.
Yeo JH, Skinner JP, Bird MJ, Formosa LE, Zhang JG, Kluck RM, Belz GT, Chong MM
PloS one 2015;10(7):e0134326
PloS one 2015;10(7):e0134326
Quality control of mitochondrial protein synthesis is required for membrane integrity and cell fitness.
Richter U, Lahtinen T, Marttinen P, Suomi F, Battersby BJ
The Journal of cell biology 2015 Oct 26;211(2):373-89
The Journal of cell biology 2015 Oct 26;211(2):373-89
Mutation in MRPS34 compromises protein synthesis and causes mitochondrial dysfunction.
Richman TR, Ermer JA, Davies SM, Perks KL, Viola HM, Shearwood AM, Hool LC, Rackham O, Filipovska A
PLoS genetics 2015 Mar;11(3):e1005089
PLoS genetics 2015 Mar;11(3):e1005089
Mitochondrial RNA Granules Are Centers for Posttranscriptional RNA Processing and Ribosome Biogenesis.
Antonicka H, Shoubridge EA
Cell reports 2015 Feb 17;10(6):920-932
Cell reports 2015 Feb 17;10(6):920-932
A bifunctional protein regulates mitochondrial protein synthesis.
Richman TR, Davies SM, Shearwood AM, Ermer JA, Scott LH, Hibbs ME, Rackham O, Filipovska A
Nucleic acids research 2014 May;42(9):5483-94
Nucleic acids research 2014 May;42(9):5483-94
Whole-exome sequencing identifies a mutation in the mitochondrial ribosome protein MRPL44 to underlie mitochondrial infantile cardiomyopathy.
Carroll CJ, Isohanni P, Pöyhönen R, Euro L, Richter U, Brilhante V, Götz A, Lahtinen T, Paetau A, Pihko H, Battersby BJ, Tyynismaa H, Suomalainen A
Journal of medical genetics 2013 Mar;50(3):151-9
Journal of medical genetics 2013 Mar;50(3):151-9
The mitochondrial RNA-binding protein GRSF1 localizes to RNA granules and is required for posttranscriptional mitochondrial gene expression.
Antonicka H, Sasarman F, Nishimura T, Paupe V, Shoubridge EA
Cell metabolism 2013 Mar 5;17(3):386-98
Cell metabolism 2013 Mar 5;17(3):386-98
No comments: Submit comment
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 16394-1-AP(MRPL44 antibody) at dilution of 1:500
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human ovary tumor using 16394-1-AP(MRPL44 antibody) at dilution of 1:50 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The MRPL44 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human MRPL44. This antibody recognizes human, mouse, rat antigen. The MRPL44 antibody has been validated for the following applications: ELISA, WB, IHC analysis.
