Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Other assay [2]
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Validation data
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- Product number
- MA5-25936 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SERPINE2 Monoclonal Antibody (OTI2C9)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI2C9
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references SERPINE2 Overexpression Is Associated with Poor Prognosis of Urothelial Carcinoma.
Chuang HW, Hsia KT, Liao JB, Yeh CC, Kuo WT, Yang YF
Diagnostics (Basel, Switzerland) 2021 Oct 18;11(10)
Diagnostics (Basel, Switzerland) 2021 Oct 18;11(10)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SERPINE2 in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with SERPINE2 (Product # MA5-25936) monoclonal antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SERPINE2 in HepG2, HeLa, SVT2, A549, COS7, Jurkat, MDCK, PC12 and MCF7 cells using 35 µg per lane. Samples were separated by SDS-PAGE and probed with SERPINE2 (Product # MA5-25936) monoclonal antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 SERPINE2 was upregulated in urothelial carcinoma tissues. Quantification of SERPINE2 immunoexpression in 75 paired urothelial carcinoma specimens and significance determined by the paired t -test. ***, p < 0.001.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 Representative sections of the normal urothelium ( A ), low-grade urothelial carcinoma (UC) ( B ), and high-grade UC ( C , D ) stained with H&E and immunostained for SERPINE2 ( E - H ). SERPINE2 immunoreactivity to the normal urothelium, low-grade UC, and high-grade UC was observed as ( E ) negative, ( F ) weak (1+), ( G ) moderate (2+), and ( H ) strong (3+). Scale bar in ( A ), 100 um. The scale bar applies to all panels.