MA5-25936

antibody from Invitrogen Antibodies

Targeting: SERPINE2 GDN, nexin, PI7, PN1, PNI

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-25936

Provider

Invitrogen Antibodies

Product name

SERPINE2 Monoclonal Antibody (OTI2C9)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

OTI2C9

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

SERPINE2 Overexpression Is Associated with Poor Prognosis of Urothelial Carcinoma.

Chuang HW, Hsia KT, Liao JB, Yeh CC, Kuo WT, Yang YF
Diagnostics (Basel, Switzerland) 2021 Oct 18;11(10)

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SERPINE2 in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with SERPINE2 (Product # MA5-25936) monoclonal antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SERPINE2 in HepG2, HeLa, SVT2, A549, COS7, Jurkat, MDCK, PC12 and MCF7 cells using 35 µg per lane. Samples were separated by SDS-PAGE and probed with SERPINE2 (Product # MA5-25936) monoclonal antibody.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry was performed on paraffin-embedded carcinoma of human kidney tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a SERPINE2 monoclonal antibody (Product # MA5-25936).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry was performed on paraffin-embedded adenocarcinoma of human endometrium tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a SERPINE2 monoclonal antibody (Product # MA5-25936).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry was performed on paraffin-embedded adenocarcinoma of human ovary tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a SERPINE2 monoclonal antibody (Product # MA5-25936).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2 SERPINE2 was upregulated in urothelial carcinoma tissues. Quantification of SERPINE2 immunoexpression in 75 paired urothelial carcinoma specimens and significance determined by the paired t -test. ***, p < 0.001.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 3 Representative sections of the normal urothelium ( A ), low-grade urothelial carcinoma (UC) ( B ), and high-grade UC ( C , D ) stained with H&E and immunostained for SERPINE2 ( E - H ). SERPINE2 immunoreactivity to the normal urothelium, low-grade UC, and high-grade UC was observed as ( E ) negative, ( F ) weak (1+), ( G ) moderate (2+), and ( H ) strong (3+). Scale bar in ( A ), 100 um. The scale bar applies to all panels.