Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- NB120-9287AF488 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Cytokeratin 8 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified. Recognizes most of the basic (Type II) keratins, HMW cytokeratins. It is broadly reactive and stains positively almost all epithelia and their neoplasms. AE3 reacts with all layers of epidermis (basal layer and above). The antibody is an excellent marker for distinguishing carcinomas from non-epithelial tumours. It has been used for studying the immunolocalisation of epidermal keratins, tissue distribution of keratins, various diseases of epidermis, oral mucosa and other epithelia and other epithelia development.
- Reactivity
- Human, Mouse, Rat, Bovine, Chicken/Avian, Rabbit, Simian
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C in the dark.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Flow Cytometry: Cytokeratin 8 Antibody (AE3) [Alexa Fluor® 488] [NB120-9287AF488] - An intracellular stain was performed on A431 cells with Cytokeratin 8 Antibody [AE3] NB120-9287AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.