GTX112975

antibody from GeneTex

Targeting: KRT8 CARD2, CK8, CYK8, K2C8, K8, KO

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX112975

Provider

GeneTex

Proper citation

GeneTex Cat#GTX112975, RRID:AB_1950655

Product name

Cytokeratin 8 antibody [N1C1]

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

References

Submitted references

Proteomic analysis of evodiamine-induced cytotoxicity in thyroid cancer cells.

Yu HI, Chou HC, Su YC, Lin LH, Lu CH, Chuang HH, Tsai YT, Liao EC, Wei YS, Yang YT, Lee YR, Chan HL
Journal of pharmaceutical and biomedical analysis 2018 Oct 25;160:344-350

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Hyaluronic acid-dependent protection against UVB-damaged human corneal cells.

Li JM, Chou HC, Wang SH, Wu CL, Chen YW, Lin ST, Chen YH, Chan HL
Environmental and molecular mutagenesis 2013 Jul;54(6):429-49

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Proteomic analysis of retinopathy-related plasma biomarkers in diabetic patients.

Lu CH, Lin ST, Chou HC, Lee YR, Chan HL
Archives of biochemistry and biophysics 2013 Jan 15;529(2):146-56

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Amniotic fluid proteome analysis from Down syndrome pregnancies for biomarker discovery.

Cho CK, Smith CR, Diamandis EP
Journal of proteome research 2010 Jul 2;9(7):3574-82

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (50 ?g of whole cell lysate) A: mouse liver 10% SDS PAGE GTX112975 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ug of whole cell lysate) A: H1299 10% SDS PAGE GTX112975 diluted at 1:1000

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Cytokeratin 8 antibody [N1C1] detects Cytokeratin 8 protein by western blot analysis.A. 30 ?g H1299 whole cell lysate/extract10% SDS-PAGECytokeratin 8 antibody [N1C1] (GTX112975) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Rat tissue extract (50 ?g) was separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 8 antibody [N1C1] (GTX112975) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Wild-type (WT) and Cytokeratin 8 knockout (KO) HeLa cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 8 antibody [N1C1] (GTX112975) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Wild-type (WT) and Cytokeratin 8 knockout (KO) HeLa cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 8 antibody [N1C1] (GTX112975) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Cytokeratin 8(GTX112975) antibody at 1:200 dilution.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using Cytokeratin 8(GTX112975) antibody at 1:500 dilution.