Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-36799 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-Cytokeratin 8 (Ser74) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody detects endogenous protein at a molecular weight of 55 kDa. Purity is >95% by SDS-PAGE.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-Cytokeratin 8 pSer74 using Phospho-Cytokeratin 8 pSer74 polyclonal antibody (Product # PA5-36799) at a dilution of 1:500. Lane 1: Hela cell lysate, Lane 2: NIH-3T3 cell lysate, Lane 3: H9C2 cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-Cytokeratin 8 (Ser74) in Lane 1: HeLa cell lysate, Lane 2: NIH-3T3 cell lysate, Lane 3: H9C2 cell lysate. Samples were incubated with Phospho-Cytokeratin 8 (Ser74) polyclonal antibody (Product # PA5-36799) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Phospho-Cytokeratin 8 (Ser74) in paraffin-embedded human lung carcinoma tissue (cytoplasmic and nucleus staining) and negative control (right, with PBS only). Samples were incubated with Phospho-Cytokeratin 8 (Ser74) polyclonal antibody (Product # PA5-36799) at a dilution of 1:50, followed by goat Anti-Rabbit IgG-biotin and avidin peroxidase.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Phospho-Cytokeratin 8 (Ser74) in paraffin-embedded human liver carcinoma tissue (cytoplasmic and nucleus staining) and negative control (right, with PBS only). Samples were incubated with Phospho-Cytokeratin 8 (Ser74) polyclonal antibody (Product # PA5-36799) at a dilution of 1:50, followed by goat Anti-Rabbit IgG-biotin and avidin peroxidase.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Phospho-Cytokeratin 8 (Ser74) in paraffin-embedded human colon carcinoma tissue (cytoplasmic and nucleus staining) and negative control (right, with PBS only). Samples were incubated with Phospho-Cytokeratin 8 (Ser74) polyclonal antibody (Product # PA5-36799) at a dilution of 1:50, followed by goat Anti-Rabbit IgG-biotin and avidin peroxidase.