IC3165G

antibody from R&D Systems

Targeting: KRT8 CARD2, CK8, CYK8, K2C8, K8, KO

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

IC3165G

Provider

R&D Systems

Product name

Human Cytokeratin 8 Alexa Fluor® 488-conjugated Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human Cytokeratin 8 in flow cytometry.

Reactivity

Human

Host

Mouse

Conjugate

Green dye

Isotype

IgG

Antibody clone number

LP3K

Vial size

100 Tests

Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Cytokeratin 8 in HT-29 Human Cell Line by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) (upper panel, negative control) and HT-29 human colon adenocarcinoma cell line (lower panel, positive control) were stained with Mouse Anti-Human Cytokeratin 8 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC3165G, filled histograms) or isotype control antibody (Catalog # IC002G, open histograms). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.