Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- ARG51782 - Provider product page
- Provider
- Arigo
- Product name
- anti-Keratin 8 phospho (Ser74) antibody
- Antibody type
- Polyclonal
- Antigen
- Peptide sequence around phosphorylation site of serine 74 (L-L-S(p)-P-L) derived from Human Keratin 8 (CK8).
- Description
- Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
- Storage
- Aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
No comments: Submit comment
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: extracts from HepG2 cells untreated or treated with EGF stained with anti-Keratin 8 (phospho Ser74) antibody ARG51782.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: extracts from 293 cells, treated with calf intestinal phosphatase (CIP), stained with anti-Keratin 8 (phospho Ser74) antibody ARG51782.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: methanol-fixed HeLa cells stained with anti-Keratin 8 (phospho Ser74) antibody ARG51782.
