Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- 904804 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#904804, RRID:AB_2616821
- Product name
- Purified anti-Cytokeratin 8
- Antibody type
- Monoclonal
- Antigen
- This antibody was raised against the 12 carboxy-terminal amino acids of HK-8 preceded by a cysteine, CKLVSESSDVLPK.
- Reactivity
- Human, Mouse, Canine, Simian
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 1.00E+08
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
- Main image
- Experimental details
- IHC staining of anti-cytokeratin 8 antibody (clone 1E8) on formalin-fixed paraffin-embedded normal human skin. Following antigen retrieval using sodium citrate, the tissue was incubated with the primary antibody at 1 µg/ml for 60 minutes at room temperature. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit was used for detection followed by hematoxylin counterstaining, according to the protocol provided.
- Conjugate
- Unconjugated