H00003856-M02

antibody from Abnova Corporation

Targeting: KRT8 CARD2, CK8, CYK8, K2C8, K8, KO

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00003856-M02

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00003856-M02, RRID:AB_1111928

Product name

KRT8 monoclonal antibody (M02), clone 3E3

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant KRT8.

Antigen sequence

EKEQIKTLNNKFASFIDKVRFLEQQNKMLETKWSL
LQQQKTARSNMDNMFESYINNLRRQLETLGQEKLK
LEAELGNMQGLVEDFKNKYEDEINKRTEMENEFVL


Isotype

IgG

Antibody clone number

3E3

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of KRT8 expression in transfected 293T cell line by KRT8 monoclonal antibody (M02), clone 3E3.Lane 1: KRT8 transfected lysate(53.7 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged KRT8 is approximately 1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to KRT8 on HeLa cell . [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol