Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- M01421-3 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Cytokeratin 8 KRT8 Antibody Picoband™ (monoclonal, 3G9)
- Antibody type
- Monoclonal
- Description
- Mouse IgG monoclonal antibody for Cytokeratin 8 detection. Tested with WB, IHC-P, IHC-F, ICC/IF, IF, FCM in Human;Mouse;Rat.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 3G9
- Vial size
- 100μg/vial
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
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Supportive validation
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- Boster Biological Technology (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of A549 cells using anti-Cytokeratin 8 antibody (M01421-3). Overlay histogram showing A549 cells stained with M01421-3 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Cytokeratin 8 Antibody (M01421-3, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
- Additional image