Ab099041

antibody from Aladdin Scientific

Targeting: KRT8 CARD2, CK8, CYK8, K2C8, K8, KO

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

Ab099041

Provider

Aladdin Scientific

Product name

Cytokeratin 8 Antibody

Antibody type

Polyclonal

Description

Rabbit anti Human Cytokeratin 8 Antibody, Polyclonal, could be used for WB, IHC, ICC/IF and so on.ApplicationWB: 0.01-2 µg/mLIHC: 5-20 µg/mLICC/IF: 5-20 µg/mLProtein FunctionTogether with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

AA 92-393


Vial size

100_l,10_l,1ml,20_l,50_l

Concentration

Lot by Lot

Storage

Store at 4_ short term (1-2 weeks). Store at -20_ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Western blot

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Cytokeratin 8 Antibody (Ab099041) - Western Blot All lanes: Cytokeratin 8 Antibody (Ab099041) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 55 kDa Observed band size: 37-55 kDa Exposure time: 0.3 s

Immunocytochemistry

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Cytokeratin 8 Antibody (Ab099041) - IF/ICC FITC staining on IF Samples: Human MCF7 cells Primary Ab: 20 μg/mL Cytokeratin 8 Antibody (Ab099041) Second Ab: 1.5 μg/mL FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

Immunohistochemistry

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Cytokeratin 8 Antibody (Ab099041) - IHC DAB staining on IHC Samples: Human Liver cancer Tissue Primary Ab: 20 µg/mL Cytokeratin 8 Antibody (Ab099041) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Cytokeratin 8 Antibody (Ab099041) - IHC DAB staining on IHC Samples: Human Tonsil Tissue Primary Ab: 10 µg/mL Cytokeratin 8 Antibody (Ab099041) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Cytokeratin 8 Antibody (Ab099041) - IHC DAB staining on IHC Samples: Human Prostate Tissue Primary Ab: 10 µg/mL Cytokeratin 8 Antibody (Ab099041) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Cytokeratin 8 Antibody (Ab099041) - IHC DAB staining on IHC Samples: Human Thyroid cancer Tissue Primary Ab: 10 µg/mL Cytokeratin 8 Antibody (Ab099041) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Cytokeratin 8 Antibody (Ab099041) - IHC DAB staining on IHC Samples: Human Kidney Tissue Primary Ab: 10 µg/mL Cytokeratin 8 Antibody (Ab099041) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Cytokeratin 8 Antibody (Ab099041) - IHC DAB staining on IHC Samples: Human Stomach Tissue Primary Ab: 10 µg/mL Cytokeratin 8 Antibody (Ab099041) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.