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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- NBP2-14177 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal Cytokeratin 8 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human Cytokeratin 8 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cytokeratin 8 Antibody [NBP2-14177] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG spLane 4: Human plasma (IgG/HSA depleted)Lane 5: Human liver tissueLane 6: Human tonsil tissue
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cytokeratin 8 Antibody [NBP2-14177] - Analysis in human cell lines MCF-7 and SK-MEL-30 using anti-KRT8 antibody. Corresponding KRT8 RNA-seq data are presented for the same cell lines. Loading control: anti-PFN1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody [NBP2-14177] - Staining of human stomach, upper shows strong cytoplasmic and membranous positivity in glandular cells.
