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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- MA5-14476 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cytokeratin 8 Monoclonal Antibody (EP1628Y)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- MA5-14476 targets Cytokeratin 8 in IHC (P) applications and shows reactivity with Human samples. The MA5-14476 immunogen is a synthetic peptide corresponding to residues of the human CK18.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- EP1628Y
- Vial size
- 1 mL
- Concentration
- Conc. Not Determined
- Storage
- 4° C
Submitted references Expression of keratins 8, 18, and 19 in epithelia of atrophic oral lichen planus.
Quantitative Image Analysis of Epithelial and Stromal Area in Histological Sections of Colorectal Cancer: An Emerging Diagnostic Tool.
Glucose and SIRT2 reciprocally mediate the regulation of keratin 8 by lysine acetylation.
Characterization of the expression of cytokeratins 5, 8, and 14 in mouse thymic epithelial cells during thymus regeneration following acute thymic involution.
Schreurs O, Karatsaidis A, Balta MG, Grung B, Hals EKB, Schenck K
European journal of oral sciences 2020 Feb;128(1):7-17
European journal of oral sciences 2020 Feb;128(1):7-17
Quantitative Image Analysis of Epithelial and Stromal Area in Histological Sections of Colorectal Cancer: An Emerging Diagnostic Tool.
Rogojanu R, Thalhammer T, Thiem U, Heindl A, Mesteri I, Seewald A, Jäger W, Smochina C, Ellinger I, Bises G
BioMed research international 2015;2015:569071
BioMed research international 2015;2015:569071
Glucose and SIRT2 reciprocally mediate the regulation of keratin 8 by lysine acetylation.
Snider NT, Leonard JM, Kwan R, Griggs NW, Rui L, Omary MB
The Journal of cell biology 2013 Feb 4;200(3):241-7
The Journal of cell biology 2013 Feb 4;200(3):241-7
Characterization of the expression of cytokeratins 5, 8, and 14 in mouse thymic epithelial cells during thymus regeneration following acute thymic involution.
Lee EN, Park JK, Lee JR, Oh SO, Baek SY, Kim BS, Yoon S
Anatomy & cell biology 2011 Mar;44(1):14-24
Anatomy & cell biology 2011 Mar;44(1):14-24
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Cytokeratin 8 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Cytokeratin 8 Monoclonal Antibody (Product # MA5-14476) at 5µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded human colon adenocarcinoma stained with Keratin 8 using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining.
