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- Product number
- PA5-79276 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FMO4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 500 µg/mL
- Storage
- -20°C
Submitted references Flavin-Containing Monooxygenases Are Conserved Regulators of Stress Resistance and Metabolism.
Huang S, Howington MB, Dobry CJ, Evans CR, Leiser SF
Frontiers in cell and developmental biology 2021;9:630188
Frontiers in cell and developmental biology 2021;9:630188
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- FIGURE 1 C. elegans FMO-2 and mammalian FMOs improve stress resistance to oxidative stress. (A) Amino acid sequence alignment of FMOs across species with the regions containing the eight essential residues denoted as red arrowheads in the catalytic active site among reconstructed ancestral mammalian FMO5, C. elegans FMO-2, and mouse FMO1-5. (B) Wild-Type and FMO-2 overexpressing (FMO-2 OE) worm survival curves on paraquat stress. Each strain was placed on NGM (Nematode Growth Medium) containing 5 mM paraquat from the fourth larvae stage (L4) at Day 0. Survival was quantified every day until all worms were dead. The difference between the survival curves is denoted with the log-rank test p -value. (C) FMO1-5 protein levels in FMO1-5 OE and control cells of both HEK293A and HepG2. (D) FMO5 OE and control cell survival curves on paraquat stress in HEK293A and HepG2. HEK293A cells or HepG2 cells stably expressing FMO5 or empty vector pDEST were subjected to indicated increasing doses of paraquat. (E) LD 50 values of FMO1-5 OE cells compared to the control cells on paraquat stress in HEK293A and HepG2. HEK293A cells or HepG2 cells stably expressing FMO1-5 or empty vector pDEST were subjected to indicated increasing doses of paraquat. (F) FMO5 OE and control cell survival curves on cadmium stress in HEK 293A and HepG2. HEK293A cells or HepG2 cells stably expressing FMO5 or empty vector pDEST were subjected to indicated increasing doses of cadmium. (G) LD 50 values of FMO-OE cells c
