Explore
Validate
Learn
Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- H00004653-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00004653-M01, RRID:AB_2148654
- Product name
- Mouse Monoclonal Myocilin Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. MYOC - myocilin, trabecular meshwork inducible glucocorticoid response
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 50 ug
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Glucocorticoid Receptor Transactivation Is Required for Glucocorticoid-Induced Ocular Hypertension and Glaucoma.
Glucocorticoid receptor GRĪ² regulates glucocorticoid-induced ocular hypertension in mice.
Patel GC, Millar JC, Clark AF
Investigative ophthalmology & visual science 2019 May 1;60(6):1967-1978
Investigative ophthalmology & visual science 2019 May 1;60(6):1967-1978
Glucocorticoid receptor GRĪ² regulates glucocorticoid-induced ocular hypertension in mice.
Patel GC, Liu Y, Millar JC, Clark AF
Scientific reports 2018 Jan 16;8(1):862
Scientific reports 2018 Jan 16;8(1):862
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Myocilin Antibody (4F8) [H00004653-M01] - Analysis of MYOC over-expressed 293 cell line, cotransfected with MYOC Validated Chimera RNAi ( Cat # H00004653-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MYOC monoclonal antibody (M01), clone 4F8 (Cat # H00004653-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Myocilin Antibody (4F8) [H00004653-M01] - Analysis of MYOC expression in transfected 293T cell line by MYOC monoclonal antibody (M01), clone 4F8.Lane 1: MYOC transfected lysate(57 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Myocilin Antibody (4F8) [H00004653-M01] - Detection limit for recombinant GST tagged MYOC is approximately 0.3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Myocilin Antibody (4F8) [H00004653-M01] - Analysis of MYOC transfected lysate using anti-MYOC monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MYOC MaxPab rabbit polyclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Myocilin Antibody (4F8) [H00004653-M01] - Analysis of monoclonal antibody to MYOC on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.
