AF2537

antibody from R&D Systems

Targeting: MYOC GLC1A, JOAG1, TIGR

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF2537

Provider

R&D Systems

Product name

Human Myocilin Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human Myocilin in direct ELISAs and Western blots.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Antigen sequence

Q99972


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Mutant myocilin impacts sarcomere ultrastructure in mouse gastrocnemius muscle.

Lynch JM, Dolman AJ, Guo C, Dolan K, Xiang C, Reda S, Li B, Prasanna G
PloS one 2018;13(11):e0206801

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Binding of a glaucoma-associated myocilin variant to the αB-crystallin chaperone impedes protein clearance in trabecular meshwork cells.

Lynch JM, Li B, Katoli P, Xiang C, Leehy B, Rangaswamy N, Saenz-Vash V, Wang YK, Lei H, Nicholson TB, Meredith E, Rice DS, Prasanna G, Chen A
The Journal of biological chemistry 2018 Dec 28;293(52):20137-20156

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Reduced myocilin expression in cultured monkey trabecular meshwork cells induced by a selective glucocorticoid receptor agonist: comparison with steroids.

Pfeffer BA, DeWitt CA, Salvador-Silva M, Cavet ME, López FJ, Ward KW
Investigative ophthalmology & visual science 2010 Jan;51(1):437-46

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Substrate rigidity modulates cell matrix interactions and protein expression in human trabecular meshwork cells.

Schlunck G, Han H, Wecker T, Kampik D, Meyer-ter-Vehn T, Grehn F
Investigative ophthalmology & visual science 2008 Jan;49(1):262-9

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human Myocilin by Western Blot. Western blot shows lysates of human heart tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Myocilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2537) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for Myocilin at approximately 55 and 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human Myocilin by Simple Western^TM. Simple Western lane view shows lysates of human heart tissue, loaded at 0.2 mg/mL. A specific band was detected for Myocilin at approximately 58 kDa (as indicated) using 50 µg/mL of Goat Anti-Human Myocilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2537) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.