Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF1054 - Provider product page
- Provider
- R&D Systems
- Product name
- Human OX40 Ligand/TNFSF4 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human OX40 Ligand/TNFSF4 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse OX40 Ligand/TNFSF4 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q6FGS4
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references HDAC11 plays an essential role in regulating OX40 ligand expression in Hodgkin lymphoma.
Mapping the domains of CD134 as a functional receptor for feline immunodeficiency virus.
Buglio D, Khaskhely NM, Voo KS, Martinez-Valdez H, Liu YJ, Younes A
Blood 2011 Mar 10;117(10):2910-7
Blood 2011 Mar 10;117(10):2910-7
Mapping the domains of CD134 as a functional receptor for feline immunodeficiency virus.
Willett BJ, McMonagle EL, Bonci F, Pistello M, Hosie MJ
Journal of virology 2006 Aug;80(15):7744-7
Journal of virology 2006 Aug;80(15):7744-7
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- IL-8 Secretion Induced by OX40 Ligand/TNFSF4 and Neutralization by Human OX40 Ligand/TNFSF4 Antibody. Recombinant Human OX40 Ligand/TNFSF4 (Catalog # 1054-OX) induces IL-8 secretion in the HT1080 human fibrosarcoma cell line transfected with human OX40 in a dose-dependent manner as measured by the Human IL-8 DuoSet (Catalog # DY208). IL-8 secretion elicited by Recombinant Human OX40 Ligand/TNFSF4 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human OX40 Ligand/TNFSF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1054). The ND50 is typically 0.015-0.09 µg/mL.