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Western blot
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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- HPA031657 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA031657, RRID:AB_10602196
- Product name
- Anti-ANKRD45
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human ANKRD45, Gene description: ankyrin repeat domain 45, Alternative Gene Names: CT117, FLJ45235, Validated applications: IHC, WB, Uniprot ID: Q5TZF3, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.1 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Profiling cancer testis antigens in non–small-cell lung cancer
Djureinovic D, Hallström B, Horie M, Mattsson J, La Fleur L, Fagerberg L, Brunnström H, Lindskog C, Madjar K, Rahnenführer J, Ekman S, Ståhle E, Koyi H, Brandén E, Edlund K, Hengstler J, Lambe M, Saito A, Botling J, Pontén F, Uhlén M, Micke P
JCI Insight 2016;1(10)
JCI Insight 2016;1(10)
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Recombinant expression validation
- Main image
- Experimental details
- Western blot analysis in control (vector only transfected HEK293T lysate) and ANKRD45 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY404885).
- Sample type
- Human
- Protocol
- Protocol
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Orthogonal validation
- Main image
- Experimental details
- Immunohistochemistry analysis in human fallopian tube and prostate tissues using Anti-ANKRD45 antibody. Corresponding ANKRD45 RNA-seq data are presented for the same tissues.
- Sample type
- Human
- Protocol
- Protocol
