Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- MAB933 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal ACE-2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human ACE-2 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) ACE-1 or rhNeprilysin is observed.
- Reactivity
- Human, Hamster
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Angiotensin-converting enzyme 2 is a potential therapeutic target for EGFR-mutant lung adenocarcinoma.
TMPRSS2 and ADAM17 cleave ACE2 differentially and only proteolysis by TMPRSS2 augments entry driven by the severe acute respiratory syndrome coronavirus spike protein.
Selective and specific regulation of ectodomain shedding of angiotensin-converting enzyme 2 by tumor necrosis factor alpha-converting enzyme.
Angiotensin converting enzyme-2 confers endothelial protection and attenuates atherosclerosis.
JNK and PI3k/Akt signaling pathways are required for establishing persistent SARS-CoV infection in Vero E6 cells.
Susceptibility to SARS coronavirus S protein-driven infection correlates with expression of angiotensin converting enzyme 2 and infection can be blocked by soluble receptor.
Yamaguchi M, Hirai S, Sumi T, Tanaka Y, Tada M, Nishii Y, Hasegawa T, Uchida H, Yamada G, Watanabe A, Takahashi H, Sakuma Y
Biochemical and biophysical research communications 2017 Jun 3;487(3):613-618
Biochemical and biophysical research communications 2017 Jun 3;487(3):613-618
TMPRSS2 and ADAM17 cleave ACE2 differentially and only proteolysis by TMPRSS2 augments entry driven by the severe acute respiratory syndrome coronavirus spike protein.
Heurich A, Hofmann-Winkler H, Gierer S, Liepold T, Jahn O, Pöhlmann S
Journal of virology 2014 Jan;88(2):1293-307
Journal of virology 2014 Jan;88(2):1293-307
Selective and specific regulation of ectodomain shedding of angiotensin-converting enzyme 2 by tumor necrosis factor alpha-converting enzyme.
Iwata M, Silva Enciso JE, Greenberg BH
American journal of physiology. Cell physiology 2009 Nov;297(5):C1318-29
American journal of physiology. Cell physiology 2009 Nov;297(5):C1318-29
Angiotensin converting enzyme-2 confers endothelial protection and attenuates atherosclerosis.
Lovren F, Pan Y, Quan A, Teoh H, Wang G, Shukla PC, Levitt KS, Oudit GY, Al-Omran M, Stewart DJ, Slutsky AS, Peterson MD, Backx PH, Penninger JM, Verma S
American journal of physiology. Heart and circulatory physiology 2008 Oct;295(4):H1377-84
American journal of physiology. Heart and circulatory physiology 2008 Oct;295(4):H1377-84
JNK and PI3k/Akt signaling pathways are required for establishing persistent SARS-CoV infection in Vero E6 cells.
Mizutani T, Fukushi S, Saijo M, Kurane I, Morikawa S
Biochimica et biophysica acta 2005 Jun 30;1741(1-2):4-10
Biochimica et biophysica acta 2005 Jun 30;1741(1-2):4-10
Susceptibility to SARS coronavirus S protein-driven infection correlates with expression of angiotensin converting enzyme 2 and infection can be blocked by soluble receptor.
Hofmann H, Geier M, Marzi A, Krumbiegel M, Peipp M, Fey GH, Gramberg T, Pöhlmann S
Biochemical and biophysical research communications 2004 Jul 9;319(4):1216-21
Biochemical and biophysical research communications 2004 Jul 9;319(4):1216-21
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human ACE-2 by Western Blot. Western blot shows lysates of NS0 mouse myeloma cell line and human kidney tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ACE-2 Monoclonal Antibody (Catalog # MAB933) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ACE-2 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ACE-2 in Human Kidney. ACE-2 in Human Kidney.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ACE‑2 in Hamster Lung.