Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [9]
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Validation data
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- Product number
- PA5-88732 - Provider product page

- Provider
- Invitrogen Antibodies
- Product name
- ACE2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Positive Samples: 293T, A-549, HeLa, Mouse kidney; Cellular Location: Cell membrane, Cytoplasm, Single-pass type I membrane protein Immunogen sequence: QSTIEEQAKT FLDKFNHEAE DLFYQSSLAS WNYNTNITEE NVQNMNNAGD KWSAFLKEQS TLAQMYPLQE IQNLTVKLQL QALQQNGSSV LSEDKSKRLN TILNTMSTIY STGKVCNPDN PQECLLLEPG LNEIMANSLD YNERLWAWES WRSEVGKQLR PLYEEYVVLK NEMARANHYE DYGDYWRGDY EVNGVDGYDY SRGQLIEDVE HTFEEIKPLY EHLHAYVRAK LMNAYPSYIS PIGCLPAHLL GDMWGRFWTN LYSLTVPFGQ KPNIDVTDAM VDQAWDAQRI FKEAEKFFVS VGLPNMTQGF WENSMLTDPG NVQKAVCHPT AWDLGKGDFR ILMCTKVTMD DFLTAHHEMG HIQYDMAYAA QPFLLRNGAN EGFHEAVGEI MSLSAATPKH LKSIGLLSPD FQEDNETEIN FLLKQALTIV GTLPFTYMLE KWRWMVFKGE IPKDQWMKKW WEMKREIVGV VEPVPHDETY CDPASLFHVS NDYSFIRYYT RTLYQFQFQE ALCQAAKHEG PLHKCDISNS TEAGQKLFNM LRLGKSEPWT LALENVVGAK NMNVRPLLNY FEPLFTWLKD QNKNSFVGWS TDWSPYADQS IKVRISLKSA LGDKAYEWND NEMYLFRSSV AYAMRQYFLK VKNQMILFGE EDVRVANLKP RISFNFFVTA PKNVSDIIPR TEVEKAIRMS RSRINDAFRL NDNSLEFLGI QPTLGPPNQP PVS
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 μL
- Concentration
- 2 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image

- Experimental details
- Western blot analysis of ACE2 in extracts of various cell lines. Samples were incubated with ACE2 Polyclonal antibody (Product # PA5-88732) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image

- Experimental details
- Immunohistochemistry analysis of ACE2 in paraffin-embedded human colon carcinoma. Samples were incubated with ACE2 Polyclonal antibody (Product # PA5-88732) using a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image

- Experimental details
- Immunohistochemistry analysis of ACE2 in paraffin-embedded mouse kidney. Samples were incubated with ACE2 Polyclonal antibody (Product # PA5-88732) using a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image

- Experimental details
- Immunohistochemistry (Immunofluorescence) analysis of ACE2 in rat testis. Samples were incubated with ACE2 Polyclonal antibody (Product # PA5-88732) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image

- Experimental details
- Immunohistochemistry (Immunofluorescence) analysis of ACE2 in rat kidney. Samples were incubated with ACE2 Polyclonal antibody (Product # PA5-88732) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image

- Experimental details
- Immunohistochemistry (Immunofluorescence) analysis of ACE2 in mouse testis. Samples were incubated with ACE2 Polyclonal antibody (Product # PA5-88732) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image

- Experimental details
- Immunohistochemistry (Immunofluorescence) analysis of ACE2 in mouse kidney. Samples were incubated with ACE2 Polyclonal antibody (Product # PA5-88732) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image

- Experimental details
- Immunohistochemistry (Immunofluorescence) analysis of ACE2 in rat testis. Samples were incubated with ACE2 Polyclonal antibody (Product # PA5-88732) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image

- Experimental details
- Immunohistochemistry (Immunofluorescence) analysis of ACE2 in mouse testis. Samples were incubated with ACE2 Polyclonal antibody (Product # PA5-88732) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image

- Experimental details
- Immunohistochemistry (Immunofluorescence) analysis of ACE2 in mouse kidney. Samples were incubated with ACE2 Polyclonal antibody (Product # PA5-88732) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.