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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [11]
- Flow cytometry [3]
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- Product number
- 66699-1-IG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ACE2 Monoclonal Antibody (2F12A4)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2F12A4
- Vial size
- 150 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Untreated and PNGase F-treated lysates of human testis tissue were subjected to SDS PAGE followed by western blot with 66699-1-IG (ACE2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66699-1-Ig (ACE2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis tissue slide using 66699-1-Ig (ACE2 antibody) at dilution of 1:300 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis tissue slide using 66699-1-Ig (ACE2 antibody) at dilution of 1:300 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human small intestine tissue slide using 66699-1-IG (ACE2 antibody) at dilution of 1:300 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human small intestine tissue slide using 66699-1-IG (ACE2 antibody) at dilution of 1:300 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using ACE2 antibody (Product # 66699-1-IG) at dilution of 1:2,000 (under 10x lens). Heat mediated antigen retrieved with Tris-EDTA buffer (pH9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using ACE2 antibody (Product # 66699-1-IG) at dilution of 1:2,000 (under 40x lens). Heat mediated antigen retrieved with Tris-EDTA buffer (pH9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon tissue slide using ACE2 antibody (Product # 66699-1-IG) at dilution of 1:2,000 (under 10x lens). Heat mediated antigen retrieved with Tris-EDTA buffer (pH9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon tissue slide using ACE2 antibody (Product # 66699-1-IG) at dilution of 1:2,000 (under 40x lens). Heat mediated antigen retrieved with Tris-EDTA buffer (pH9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using ACE2 antibody (Product # 66699-1-IG) at dilution of 1:2,000 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using ACE2 antibody (Product # 66699-1-IG) at dilution of 1:2000 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using ACE2 antibody (Product # 66699-1-IG, Clone: 2F12A4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HeLa cells were surface stained with 0.20 µg/test Anti-Human ACE2 (Product #66699-1-IG) (red) or 0.20 µg control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1,000. Fixed with 90% MeOH.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 NIH/3T3 cells were surface stained with 0.20 µg/test Anti-Human ACE2 (Product # 66699-1-IG) (red) or 0.20 µg control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1,000. Fixed with 90% MeOH.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 THP-1 cells were surface stained with 0.20 µg/test Anti-Human ACE2 (Product # 66699-1-IG) (red) or 0.20 µg control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1,000. Fixed with 90% MeOH.
