Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [8]
- Flow cytometry [1]
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Validation data
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- Product number
- NBP1-88123 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-88123, RRID:AB_11039145
- Product name
- Rabbit Polyclonal ACE-2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human ACE-2 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Variance decomposition of protein profiles from antibody arrays using a longitudinal twin model.
Gene expression profiling of metaplastic lineages identifies CDH17 as a prognostic marker in early stage gastric cancer.
Kato BS, Nicholson G, Neiman M, Rantalainen M, Holmes CC, Barrett A, Uhlén M, Nilsson P, Spector TD, Schwenk JM
Proteome science 2011 Nov 17;9:73
Proteome science 2011 Nov 17;9:73
Gene expression profiling of metaplastic lineages identifies CDH17 as a prognostic marker in early stage gastric cancer.
Lee HJ, Nam KT, Park HS, Kim MA, Lafleur BJ, Aburatani H, Yang HK, Kim WH, Goldenring JR
Gastroenterology 2010 Jul;139(1):213-25.e3
Gastroenterology 2010 Jul;139(1):213-25.e3
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ACE-2 Antibody [NBP1-88123] - Analysis in human kidney tissue.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ACE-2 Antibody [NBP1-88123] - Analysis in human kidney tissue.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: ACE-2 Antibody [NBP1-88123] - Staining of human kidney shows strong cytoplasmic positivity in renal tubules.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ACE-2 Antibody [NBP1-88123] - Staining of human liver shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ACE-2 Antibody [NBP1-88123] - Staining of human small intestine shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ACE-2 Antibody [NBP1-88123] - Staining of human small intestine shows strong positivity in apical membranes in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ACE-2 Antibody [NBP1-88123] - Staining of human kidney shows strong positivity in apical membrane in cells in tubules.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ACE-2 Antibody [NBP1-88123] - Staining of human tonsil shows no positivity in lymphoid cells as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ACE-2 Antibody [NBP1-88123] - Staining of human duodenum shows strong positivity in apical membranes in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ACE-2 Antibody [NBP1-88123] - Staining in human small intestine and tonsil tissues.. Corresponding ACE2 RNA-seq data are presented for the same tissues.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: ACE-2 Antibody [NBP1-88123] - Detection of ACE-2 in HEK293 Human Cell Line Transfected with Human ACE-2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human ACE-2 or (B) irrelevant protein, and eGFP was stained with Rabbit Anti-Human ACE-2 Polyclonal Antibody (Catalog # NBP1-88123) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). Quadrant markers were set based on Rabbit IgG control antibody staining (Catalog # MAB1050, data not shown). View our protocol for Staining Membrane-associated Proteins.