PA5-20045

antibody from Invitrogen Antibodies

Targeting: ACE2

Western blot

Antibody data

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Validation data

Product number: PA5-20045 - Provider product page
Provider: Invitrogen Antibodies
Product name: ACE2 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: In Western blot applications, this antibody detects a band at ~90kDa. A suggested positive control is human kidney tissue lysate. PA5-20045 can be used with blocking peptide PEP-0164.
Reactivity: Human
Host: Rabbit
Isotype: IgG
Vial size: 100 µg
Concentration: 1 mg/mL
Storage: 4°C

ACE2 protein structure - PA5-20045 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of 4% paraformaldehyde-fixed Caco2 cells labeling ACE2 with ACE2 Polyclonal Antibody (Product # PA5-20045) at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (green) and DAPI staining (blue). Image showing membrane staining on Caco2 cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of 4% paraformaldehyde-fixed Caco2 cells labeling ACE2 with ACE2 Polyclonal Antibody (Product # PA5-20045) at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (green) and DAPI staining (blue). Image showing membrane staining on Caco2 cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of 4% paraformaldehyde-fixed human kidney cells labeling ACE2 with ACE2 Polyclonal Antibody (Product # PA5-20045) at 10 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (red).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of 4% paraformaldehyde-fixed Caco2 cells labeling ACE2 with ACE2 Polyclonal Antibody (Product # PA5-20045) at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (green) and DAPI staining (blue). Image showing membrane staining on Caco2 cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of 4% paraformaldehyde-fixed human kidney cells labeling ACE2 with ACE2 Polyclonal Antibody (Product # PA5-20045) at 10 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (red).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded human kidney tissue using ACE2 Polyclonal Antibody (Product # PA5-20045) at 2 µg/mL. Tissue was fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of 4% paraformaldehyde-fixed human testis tissue labeling ACE-2 with ACE2 Polyclonal Antibody (Product # PA5-20045) at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (green) and DAPI staining (blue).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse lung tissue labeling ACE-2 with ACE2 Polyclonal Antibody (Product # PA5-20045) at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (green) and DAPI staining (blue).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of 4% paraformaldehyde-fixed rat lung tissue labeling ACE-2 with ACE2 Polyclonal Antibody (Product # PA5-20045) at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (green) and DAPI staining (blue).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of 4% paraformaldehyde-fixed human lung tissue labeling ACE-2 with ACE2 Polyclonal Antibody (Product # PA5-20045) at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (green) and DAPI staining (blue).