Explore
Validate
Learn
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NB100-656 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-656, RRID:AB_2179727
- Product name
- Rabbit Polyclonal Roquin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Roquin binds microRNA-146a and Argonaute2 to regulate microRNA homeostasis.
Srivastava M, Duan G, Kershaw NJ, Athanasopoulos V, Yeo JH, Ose T, Hu D, Brown SH, Jergic S, Patel HR, Pratama A, Richards S, Verma A, Jones EY, Heissmeyer V, Preiss T, Dixon NE, Chong MM, Babon JJ, Vinuesa CG
Nature communications 2015 Feb 20;6:6253
Nature communications 2015 Feb 20;6:6253
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Roquin Antibody [NB100-656] - Samples: A) Whole cell lysate from HeLa (5, 15 and 50 ug) and 293T (T, 50 ug) cells. B) Whole cell lysate (1 mg/IP; 1/4 of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Roquin antibody NB100-656 used at 0.04 ug/ml for WB (A and B) and at 3 ug/mg lysate for IP (B). Roquin was also immunoprecipitated using rabbit anti-Roquin antibody NB100-655 at 3 ug/mg lysate. Detection: Chemiluminescence with an exposure time of 30 seconds.
