Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 12-6319-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD156c (ADAM10) Monoclonal Antibody (OV1F12), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This OV1F12 (1F12HE) monoclonal antibody reacts with human CD156c, which is also known as a disintegrin and metalloproteinase domain-containing protein (ADAM) 10. This type I transmembrane glycoprotein is expressed ubiquitously on hematopoietic and non-hematopoietic cell types. Studies demonstrate that CD156c expression is regulated by PAX2 in cancer cells. CD156c plays a critical role in hematopoiesis, including lymphocyte development, and autoimmunity by regulating the shedding and secretion of various receptors and chemokines, including Notch, CD23, CXCL16, and the IL-6R. Finally, deregulated CD156c expression has been associated with T-cell acute lymphoblastic lymphoma (T-ALL) and Alzheimer's disease. Applications Reported: This OV1F12 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This OV1F12 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Antibody clone number
- OV1F12
- Vial size
- 100 Tests
- Concentration
- 5 µl/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Redundancy and specificity of the metalloprotease system mediating oncogenic NOTCH1 activation in T-ALL.
The emergence of ADAM10 as a regulator of lymphocyte development and autoimmunity.
Sulis ML, Saftig P, Ferrando AA
Leukemia 2011 Oct;25(10):1564-9
Leukemia 2011 Oct;25(10):1564-9
The emergence of ADAM10 as a regulator of lymphocyte development and autoimmunity.
Gibb DR, Saleem SJ, Chaimowitz NS, Mathews J, Conrad DH
Molecular immunology 2011 Jun;48(11):1319-27
Molecular immunology 2011 Jun;48(11):1319-27
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Normal human peripheral blood cells were fixed and permeabilized using the Fixation & Permeabilization Buffers (Product # 88-8823-88) and then stained with either Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (blue histogram) or Anti-Human CD156c (ADAM10) PE (purple histogram). Cells in the lymphocyte gate were used for analysis.
- Conjugate
- Yellow dye