Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [1]
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Validation data
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- Product number
- RQ7053 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- ADAM10 Antibody
- Antibody type
- Polyclonal
- Description
- This highly specific ADAM10 antibody is suitable for use in Western blot/Flow cytometry/Direct ELISA applications with human samples.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the ADAM10 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) RT4 and 2) A549 cell lysate with ADAM10 antibody. Predicted molecular weight: ~84 kDa (full length), ~60 kDa (active form), ~80 kDa (glycosylated active form), ~110 kDa (glycosylated full length).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human U937 cells with ADAM10 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ADAM10 antibody.