Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA1-12500 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ADAM10 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is expected to cross-react with bovine samples due to sequence homology.
- Concentration
- 1 mg/mL
Submitted references Loss of Adam10 Disrupts Ion Transport in Immortalized Kidney Collecting Duct Cells.
Behavioural effects and regulation of PKCalpha and MAPK by huprine X in middle aged mice.
Assmus A, Mullins L, Ward M, Dobie R, Hunter R, Henderson NC, Mullins JJ
Function (Oxford, England) 2021;2(4):zqab024
Function (Oxford, England) 2021;2(4):zqab024
Behavioural effects and regulation of PKCalpha and MAPK by huprine X in middle aged mice.
Ratia M, Giménez-Llort L, Camps P, Muñoz-Torrero D, Clos MV, Badia A
Pharmacology, biochemistry, and behavior 2010 Jun;95(4):485-93
Pharmacology, biochemistry, and behavior 2010 Jun;95(4):485-93
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ADAM10 using a polyclonal antibody (Product # PA1-12500).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3. Identification of 2 Clones Resulting From Targeting of the Adam10 Gene in mCCD cl1 . (A) Transfection results on mCCD cl1 cell population for the Adam10 gene. ""Del"" for deletion; WT represents the predicted size of the WT product. T7 product of G2 is a doublet. (B) T7 screening for Adam10 transfected mCCD cl1 cells populations, following single-cell cloning. Red arrows indicate the clones selected after screening, based on the disappearance of a WT-sized band. ""Ins"" for insertion; ""Del"" for deletion; WT represents the predicted size of the WT product. The numbers above each gel track are the initial identification numbers of each clone. (C) Sequencing of PCR products from both clonal cell lines for Adam10. (D) Immunostaining of mCCD cl1 cells using anti-Adam10 antibody in wild-type mCCD cl1 cells (WT) and KO clonal lines A1 and A5. Composite image with DAPI staining of cell nuclei (blue). All scale bars 30 mum.