PA1-973

antibody from Invitrogen Antibodies

Targeting: PSMD1 P112, Rpn2, S1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-973

Provider

Invitrogen Antibodies

Product name

PSMD1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

PA1-973 detects proteasome 19S subunit S1 from from human cells. PA1-973 has been successfully used in Western blot and immunohistochemistry procedures. By Western blot, this antibody detects an ~105 kDa protein representing proteasome 19S subunit S1 from HeLa cell extract. PA1-973 immunizing peptide corresponds to amino acid residues 929-953 from human proteasome 19S subunit S1 protein.

Reactivity

Human, Mouse, Rat, Bovine

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Inactive USP14 and inactive UCHL5 cause accumulation of distinct ubiquitinated proteins in mammalian cells.

Chadchankar J, Korboukh V, Conway LC, Wobst HJ, Walker CA, Doig P, Jacobsen SJ, Brandon NJ, Moss SJ, Wang Q
PloS one 2019;14(11):e0225145

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Impairments in age-dependent ubiquitin proteostasis and structural integrity of selective neurons by uncoupling Ran GTPase from the Ran-binding domain 3 of Ranbp2 and identification of novel mitochondrial isoforms of ubiquitin-conjugating enzyme E2I (ubc9) and Ranbp2.

Patil H, Yoon D, Bhowmick R, Cai Y, Cho KI, Ferreira PA
Small GTPases 2019 Mar;10(2):146-161

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Differential loss of prolyl isomerase or chaperone activity of Ran-binding protein 2 (Ranbp2) unveils distinct physiological roles of its cyclophilin domain in proteostasis.

Cho KI, Patil H, Senda E, Wang J, Yi H, Qiu S, Yoon D, Yu M, Orry A, Peachey NS, Ferreira PA
The Journal of biological chemistry 2014 Feb 21;289(8):4600-25

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Glucosamine induces cell death via proteasome inhibition in human ALVA41 prostate cancer cell.

Liu BQ, Meng X, Li C, Gao YY, Li N, Niu XF, Guan Y, Wang HQ
Experimental & molecular medicine 2011 Sep 30;43(9):487-93

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Persistent hijacking of brain proteasomes in HIV-associated dementia.

Nguyen TP, Soukup VM, Gelman BB
The American journal of pathology 2010 Feb;176(2):893-902

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The cyclophilin-like domain of Ran-binding protein-2 modulates selectively the activity of the ubiquitin-proteasome system and protein biogenesis.

Yi H, Friedman JL, Ferreira PA
The Journal of biological chemistry 2007 Nov 30;282(48):34770-8

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Reduced ubiquitin C-terminal hydrolase-1 expression levels in dementia with Lewy bodies.

Barrachina M, Castaño E, Dalfó E, Maes T, Buesa C, Ferrer I
Neurobiology of disease 2006 May;22(2):265-73

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Neuropathology and pathogenesis of encephalitis following amyloid-beta immunization in Alzheimer's disease.

Ferrer I, Boada Rovira M, Sánchez Guerra ML, Rey MJ, Costa-Jussá F
Brain pathology (Zurich, Switzerland) 2004 Jan;14(1):11-20

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Neuropathology and pathogenesis of encephalitis following amyloid-beta immunization in Alzheimer's disease.

Ferrer I, Boada Rovira M, Sánchez Guerra ML, Rey MJ, Costa-Jussá F
Brain pathology (Zurich, Switzerland) 2004 Jan;14(1):11-20

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on whole cell extracts (30 µglysate) of MCF7 (Lane 1), PC-3 (Lane 2), Hep G2 (Lane 3), SK-OV-3 (Lane 4), HeLa (Lane 5), COS-7 (Lane 6), Jurkat (Lane 7), A-431 (Lane 8) and tissue extracts (30 µglysate) of Mouse Testis (Lane 9) and Rat Testis (Lane 10). The blot was probed with Anti-PSMD1 Rabbit Polyclonal Antibody (Product # PA1-973, 1 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 106 kDa band corresponding to PSMD1 was observed across the cell lines and tissues tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with overnight wet transfer system. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of PSMD1 was done on MCF7 cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with PSMD1 Rabbit Polyclonal Antibody (PA1973, red histogram) or with rabbit isotype control (pink histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Goat Anti-Rabbit Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control..

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

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