Explore
Validate
Learn
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunoprecipitation [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-56114 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PSMD1 Monoclonal Antibody (K70027_1F5)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Sequence of this protein is as follows: LNKDLKMPKV QYKSNCKPST FAYPAPLEVP KEKEKEKVST AVLSITAKAK KKEKEKEKKE EEKMEVDEAE KKEEKEKKKE PEPNFQLLDN PARVMPAQLK VLTMPETCRY QPFKPLSIGG IIILKDTSED
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- K70027_1F5
- Vial size
- 200 μg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of PSMD1 in 200 µg of HepG2 lysate. Samples are as follows: Lane 1: HepG2 lysate, Lane 2: PSMD1 immunoprecipitated from HepG2 lysate, Lane3: The same as Lane 2 but KT82 was used as IgG isotype control antibody. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE, blotted onto nitrocellulose membrane, and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. The isotype control antibody was KT82. Incubation of samples with PSMD1 monoclonal antibody (Product # MA5-55734) at a dilution of 2.5 µg was used.
