PA1322-1

antibody from Boster Biological Technology

Targeting: NPHS2 PDCN, SRN1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

PA1322-1

Provider

Boster Biological Technology

Product name

Anti-Podocin NPHS2 Antibody

Antibody type

Polyclonal

Description

Polyclonal antibody for PODOCIN/NPHS2 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. PODOCIN/NPHS2 information: Molecular Weight: 42201 MW; Subcellular Localization: Isoform 1: Cell membrane ; Peripheral membrane protein ; Tissue Specificity: Almost exclusively expressed in the podocytes of fetal and mature kidney glomeruli.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

100μg/vial

Concentration

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

References

Submitted references

Scutellarin ameliorates diabetic nephropathy via TGF-β1 signaling pathway.

Huang B, Han R, Tan H, Zhu W, Li Y, Jiang F, Xie C, Ren Z, Shi R
Natural products and bioprospecting 2024 Apr 24;14(1):25

---

Effect of Proprotein Convertase Subtilisin/Kexin Type 9 Inhibition on Podocytes in Mouse Nephrotic Syndrome.

Suzuki T, Iyoda M, Kanazawa N, Tachibana S, Honda H
Laboratory investigation; a journal of technical methods and pathology 2023 Sep;103(9):100199

---

Scutellarin ameliorates diabetic nephropathy via TGF-β1 signaling pathway.

Huang B, Han R, Tan H, Zhu W, Li Y, Jiang F, Xie C, Shi R
Biological & pharmaceutical bulletin 2023 Oct 17;

---

Phloretin ameliorates diabetic nephropathy by inhibiting nephrin and podocin reduction through a non-hypoglycemic effect.

Liu J, Sun M, Xia Y, Cui X, Jiang J
Food & function 2022 Jun 20;13(12):6613-6622

---

Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism.

Fu Y, Sun Y, Wang M, Hou Y, Huang W, Zhou D, Wang Z, Yang S, Tang W, Zhen J, Li Y, Wang X, Liu M, Zhang Y, Wang B, Liu G, Yu X, Sun J, Zhang C, Yi F
Cell metabolism 2020 Dec 1;32(6):1052-1062.e8

---

Rhodojaponin II attenuates kidney injury by regulating TGF-β1/Smad pathway in mice with adriamycin nephropathy.

Qiu Y, Zhou J, Zhang H, Zhou H, Tang H, Lei C, Ye C, You C, Chen Y, Wang Y, Xiong J, Su H, Yao G, Zhang C
Journal of ethnopharmacology 2019 Oct 28;243:112078

---

Antioxidative Stress Effects of Salvia przewalskii Extract in Experimentally Injured Podocytes.

Liu X, Liu Y, Yang Y, Xu J, Dai D, Yan C, Li X, Tang R, Yu C, Ren H
Nephron 2016;134(4):253-271

---

Effect of simvastatin on the expression of nephrin, podocin, and vascular endothelial growth factor (VEGF) in podocytes of diabetic rat.

Chen S, Chen H, Liu Q, Ma Q
International journal of clinical and experimental medicine 2015;8(10):18225-34

---

Effect of Cordyceps sinensis and Tripterygium wilfordii polyglycosidium on podocytes in rats with diabetic nephropathy.

Hao L, Pan MS, Zheng Y, Wang RF
Experimental and therapeutic medicine 2014 Jun;7(6):1465-1470

---

Effect of arctiin on glomerular filtration barrier damage in STZ-induced diabetic nephropathy rats.

Ma ST, Liu DL, Deng JJ, Niu R, Liu RB
Phytotherapy research : PTR 2013 Oct;27(10):1474-80

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of NPHS2 using anti- NPHS2 antibody (PA1322-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- NPHS2 antigen affinity purified polyclonal antibody (Catalog # PA1322-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NPHS2 at approximately 45KD. The expected band size for NPHS2 is at 42KD.

Additional image