HPA049486

antibody from Atlas Antibodies

Targeting: NPHS2 PDCN, SRN1

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA049486

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA049486, RRID:AB_2680788

Product name

Anti-NPHS2

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

KAASESLRMAAEILSGTPAAVQLRYLHTLQSLSTE
KPSTVVLPLPFDLLNCLSSPSNRTQGSLPFPSPSK
PVEPLNPK


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human kidney and pancreas tissues using Anti-NPHS2 antibody. Corresponding NPHS2 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human pancreas shows low expression as expected.

Sample type

HUMAN