20384-1-AP

antibody from Proteintech Group

Targeting: NPHS2 PDCN, SRN1

Provider product page for 20384-1-AP

Western blot

ELISA

Immunocytochemistry

Antibody data

Antibody Data
Antigen structure
References [49]
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Validations
Western blot [1]

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Product number: 20384-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#20384-1-AP, RRID:AB_10697662
Product name: NPHS2 antibody
Antibody type: Polyclonal
Description: NPHS2 antibody (Cat. #20384-1-AP) is a rabbit polyclonal antibody that shows reactivity with mouse, rat and has been validated for the following applications: IF, WB,ELISA.
Reactivity: Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

NPHS2 protein structure - 20384-1-AP shown in red.

Submitted references Effectiveness of a novel rat model of off-target PLA2R1 knockout to renal impairment.

Huang B, Zhang Z, Sui W, Zhao L, Li Y, Feng L, Yang D, Zhou Y
Genomics 2024 Mar;116(2):110796

Rutaecarpine protects podocytes in diabetic kidney disease by targeting VEGFR2/NLRP3-mediated pyroptosis.

Hu X, Wang J, Jiang L, Liu X, Ge Q, Wang Q, Qi X, Wu Y
International immunopharmacology 2024 Mar 30;130:111790

Placenta-derived mesenchymal stem cells protect against diabetic kidney disease by upregulating autophagy-mediated SIRT1/FOXO1 pathway.

Liu H, Wang J, Yue G, Xu J
Renal failure 2024 Dec;46(1):2303396

A small molecule chaperone rescues keratin-8 mediated trafficking of misfolded podocin to correct genetic Nephrotic Syndrome.

Kuzmuk V, Pranke I, Rollason R, Butler M, Ding WY, Beesley M, Waters AM, Coward RJ, Sessions R, Tuffin J, Foster RR, Mollet G, Antignac C, Edelman A, Welsh GI, Saleem MA
Kidney international 2024 Apr;105(4):744-758

Unraveling the interplay of ferroptosis and immune dysregulation in diabetic kidney disease: a comprehensive molecular analysis.

Jiao Y, Liu X, Shi J, An J, Yu T, Zou G, Li W, Zhuo L
Diabetology & metabolic syndrome 2024 Apr 20;16(1):86

The influence of angiopoietin-like protein 3 on macrophages polarization and its effect on the podocyte EMT in diabetic nephropathy.

Ma Y, Chen Y, Xu H, Du N
Frontiers in immunology 2023;14:1228399

Mapping of the podocin proximity-dependent proteome reveals novel components of the kidney podocyte foot process.

Gerlach GF, Imseis ZH, Cooper SL, Santos AN, O'Brien LL
Frontiers in cell and developmental biology 2023;11:1195037

Minnelide combined with anti-ANGPTL3-FLD monoclonal antibody completely protects mice with adriamycin nephropathy by promoting autophagy and inhibiting apoptosis.

Ji B, Liu J, Yin Y, Xu H, Shen Q, Yu J
Cell death & disease 2023 Sep 9;14(9):601

Irisin ameliorates diabetic kidney disease by restoring autophagy in podocytes.

Lai W, Luo D, Li Y, Li Y, Wang Q, Hu Z, Ye Z, Peng H
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2023 Oct;37(10):e23175

Klotho inhibits renal ox-LDL deposition via IGF-1R/RAC1/OLR1 signaling to ameliorate podocyte injury in diabetic kidney disease.

Jiang W, Gan C, Zhou X, Yang Q, Chen D, Xiao H, Dai L, Chen Y, Wang M, Yang H, Li Q
Cardiovascular diabetology 2023 Oct 27;22(1):293

Acteoside protects podocyte against apoptosis through regulating AKT/GSK-3β signaling pathway in db/db mice.

Li X, Liu Z, He Z, Wang X, Li R, Wang J, Ma G, Zhang P, Ma C
BMC endocrine disorders 2023 Oct 23;23(1):230

mTOR/ULK1 signaling axis mediated ultrashort wave regulation of autophagy to alleviate diabetic kidney disease.

Yan Y, Yu S, Fan Y
Advances in clinical and experimental medicine : official organ Wroclaw Medical University 2023 Nov;32(11):1265-1277

Deciphering the pharmacological mechanisms of Rostellularia procumbens (L) Nees. Extract alleviates adriamycin-induced nephropathy in vivo and in vitro.

Ai Z, Wang M, Zhou Y, Yuan D, Jian Q, Wu S, Liu B, Yang Y
Phytomedicine : international journal of phytotherapy and phytopharmacology 2023 May;113:154736

Podocyte-specific deletion of ubiquitin carboxyl-terminal hydrolase L1 causes podocyte injury by inducing endoplasmic reticulum stress.

Hu Y, Qi C, Shi J, Tan W, Adiljan Abdurusul, Zhao Z, Xu Y, Wu H, Zhang Z
Cellular and molecular life sciences : CMLS 2023 Mar 23;80(4):106

Wen-Shen-Jian-Pi-Hua-Tan decoction protects against early obesity-related glomerulopathy by improving renal bile acid composition and suppressing lipogenesis, inflammation, and fibrosis.

Song D, Zhang A, Hu X, Zeng M, Zhou H
Phytomedicine : international journal of phytotherapy and phytopharmacology 2023 Jul 25;116:154861

Minnelide combined with Angptl3 knockout completely protects mice with adriamycin nephropathy via suppression of TGF-β1-Smad2 and p53 pathways.

Ji B, Liu J, Ma Y, Yin Y, Xu H, Shen Q, Yu J
International immunopharmacology 2023 Feb;115:109656

Placental Mesenchymal Stem Cells Alleviate Podocyte Injury in Diabetic Kidney Disease by Modulating Mitophagy via the SIRT1-PGC-1alpha-TFAM Pathway.

Han X, Wang J, Li R, Huang M, Yue G, Guan L, Deng Y, Cai W, Xu J
International journal of molecular sciences 2023 Feb 28;24(5)

Qian Yang Yu Yin granule improves hypertensive renal damage: A potential role for TRPC6-CaMKKβ-AMPK-mTOR-mediated autophagy.

Ma S, Xu J, Zheng Y, Li Y, Wang Y, Li H, Fang Z, Li J
Journal of ethnopharmacology 2023 Feb 10;302(Pt A):115878

Minnelide Markedly Reduces Proteinuria in Mice with Adriamycin Nephropathy by Protecting Against Podocyte Injury.

Ji B, Liu J, Ma Y, Yin Y, Xu H, Shen Q, Yu J
Applied biochemistry and biotechnology 2023 Dec;195(12):7379-7396

Qizhi Jiangtang capsule activates podocyte autophagy in diabetic kidney disease by inhibiting phosphatidylinositol 3-kinase/protein kinase B/mammalian target of rapamycin pathways.

Zhaoan G, Lina S, Yingying L, Ruifeng LI, Chong L, Ke D, Jing S, Jun S
Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan 2023 Aug;43(4):667-675

Adeno-associated virus gene therapy prevents progression of kidney disease in genetic models of nephrotic syndrome.

Ding WY, Kuzmuk V, Hunter S, Lay A, Hayes B, Beesley M, Rollason R, Hurcombe JA, Barrington F, Masson C, Cathery W, May C, Tuffin J, Roberts T, Mollet G, Chu CJ, McIntosh J, Coward RJ, Antignac C, Nathwani A, Welsh GI, Saleem MA
Science translational medicine 2023 Aug 9;15(708):eabc8226

Peroxisome Proliferator-Activated Receptor Gene Knockout Promotes Podocyte Injury in Diabetic Mice.

Yan R, Zhang Y, Yang Y, Liu L, Liu L, Guo Z, Yu H, Wang Y, Guo B
BioMed research international 2022;2022:9018379

Canagliflozin reverses Th1/Th2 imbalance and promotes podocyte autophagy in rats with membranous nephropathy.

Lv X, Wang J, Zhang L, Shao X, Lin Y, Liu H, Ma G, Li J, Zhou S, Yu P
Frontiers in immunology 2022;13:993869

Yi-Shen-Hua-Shi Granule Alleviates Adriamycin-Induced Glomerular Fibrosis by Suppressing the BMP2/Smad Signaling Pathway.

Tan Z, Si Y, Yu Y, Ding J, Huang L, Xu Y, Zhang H, Lu Y, Wang C, Yu B, Yuan L
Frontiers in pharmacology 2022;13:917428

Effects of Shenkang Pills on Early-Stage Diabetic Nephropathy in db/db Mice via Inhibiting AURKB/RacGAP1/RhoA Signaling Pathway.

Wang F, Fan J, Pei T, He Z, Zhang J, Ju L, Han Z, Wang M, Xiao W
Frontiers in pharmacology 2022;13:781806

ManNAc protects against podocyte pyroptosis via inhibiting mitochondrial damage and ROS/NLRP3 signaling pathway in diabetic kidney injury model.

Gao Y, Ma Y, Xie D, Jiang H
International immunopharmacology 2022 Jun;107:108711

Podocyte-specific Crb2 knockout mice develop focal segmental glomerulosclerosis.

Tanoue A, Katayama K, Ito Y, Joh K, Toda M, Yasuma T, D'Alessandro-Gabazza CN, Kawachi H, Yan K, Ito M, Gabazza EC, Tryggvason K, Dohi K
Scientific reports 2021 Oct 15;11(1):20556

Experimental study on renoprotective effect of intermedin on diabetic nephropathy.

Wang Y, Tian J, Mi Y, Ren X, Lian S, Kang J, Wang J, Zang H, Wu Z, Yang J, Qiao X, Zhou X, Wang G, Zhou Y, Li R
Molecular and cellular endocrinology 2021 May 15;528:111224

Carnosine alleviates podocyte injury in diabetic nephropathy by targeting caspase-1-mediated pyroptosis.

Zhu W, Li YY, Zeng HX, Liu XQ, Sun YT, Jiang L, Xia LL, Wu YG
International immunopharmacology 2021 Dec;101(Pt B):108236

A Technique for Studying Glomerular Filtration Integrity in the Zebrafish Pronephros.

Kolatsi-Joannou M, Osborn D
Methods in molecular biology (Clifton, N.J.) 2020;2067:25-39

Adriamycin does not damage podocytes of zebrafish larvae.

Schindler M, Blumenthal A, Moeller MJ, Endlich K, Endlich N
PloS one 2020;15(11):e0242436

Paecilomyces cicadae-fermented Radix astragali activates podocyte autophagy by attenuating PI3K/AKT/mTOR pathways to protect against diabetic nephropathy in mice.

Yang F, Qu Q, Zhao C, Liu X, Yang P, Li Z, Han L, Shi X
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2020 Sep;129:110479

An integrated transcriptomics and network pharmacology approach to exploring the mechanism of adriamycin-induced kidney injury.

He S, Li A, Zhang W, Zhang L, Liu Y, Li K, Qin X
Chemico-biological interactions 2020 Jul 1;325:109096

Prolonged podocyte depletion in larval zebrafish resembles mammalian focal and segmental glomerulosclerosis.

Hansen KUI, Siegerist F, Daniel S, Schindler M, Iervolino A, Blumenthal A, Daniel C, Amann K, Zhou W, Endlich K, Endlich N
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 Dec;34(12):15961-15974

Plakoglobin is involved in cytoskeletal rearrangement of podocytes under the regulation of UCH-L1.

Fang Y, Li F, Qi C, Mao X, Xu Y, Zhao Z, Wu H, Zhang Z
Biochemical and biophysical research communications 2020 Aug 13;529(1):112-118

Exploration the active compounds of Astragali Radix in treatment of adriamycin nephropathy by network pharmacology combined with transcriptomic approach.

Li AP, He SS, Zhang WN, Zhang LC, Liu YT, Li K, Qin XM
Journal of ethnopharmacology 2020 Aug 10;258:112537

Constructing an Isogenic 3D Human Nephrogenic Progenitor Cell Model Composed of Endothelial, Mesenchymal, and SIX2-Positive Renal Progenitor Cells.

Nguyen L, Spitzhorn LS, Adjaye J
Stem cells international 2019;2019:3298432

MiR-320a induces diabetic nephropathy via inhibiting MafB.

He M, Wang J, Yin Z, Zhao Y, Hou H, Fan J, Li H, Wen Z, Tang J, Wang Y, Wang DW, Chen C
Aging 2019 May 17;11(10):3055-3079

Inhibiting 4E-BP1 re-activation represses podocyte cell cycle re-entry and apoptosis induced by adriamycin.

Li F, Mao X, Zhuang Q, Zhao Z, Zhang Z, Wu H
Cell death & disease 2019 Mar 11;10(3):241

Critical roles of PI3K/Akt/NF‑κB survival axis in angiotensin II‑induced podocyte injury.

Wang J, Fu D, Senouthai S, You Y
Molecular medicine reports 2019 Dec;20(6):5134-5144

Fractalkine is Involved in Lipopolysaccharide-Induced Podocyte Injury through the Wnt/β-Catenin Pathway in an Acute Kidney Injury Mouse Model.

Senouthai S, Wang J, Fu D, You Y
Inflammation 2019 Aug;42(4):1287-1300

Expression and distribution of PIEZO1 in the mouse urinary tract.

Dalghi MG, Clayton DR, Ruiz WG, Al-Bataineh MM, Satlin LM, Kleyman TR, Ricke WA, Carattino MD, Apodaca G
American journal of physiology. Renal physiology 2019 Aug 1;317(2):F303-F321

Weighted Gene Correlation Network Analysis (WGCNA) Detected Loss of MAGI2 Promotes Chronic Kidney Disease (CKD) by Podocyte Damage.

Zuo Z, Shen JX, Pan Y, Pu J, Li YG, Shao XH, Wang WP
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018;51(1):244-261

Nestin Improves Preeclampsia-Like Symptoms by Inhibiting Activity of Cyclin-Dependent Kinase 5.

Yang X, Ding Y, Yang M, Yu L, Hu Y, Deng Y
Kidney & blood pressure research 2018;43(2):616-627

Quercetin ameliorates podocyte injury via inhibition of oxidative stress and the TGF-β1/Smad pathway in DN rats.

Gao F, He X, Liang S, Liu S, Liu H, He Q, Chen L, Jiang H, Zhang Y
RSC advances 2018 Oct 15;8(62):35413-35421

Response of Renal Podocytes to Excessive Hydrostatic Pressure: a Pathophysiologic Cascade in a Malignant Hypertension Model.

Abu Hamad R, Berman S, Hachmo Y, Stark M, Hasan F, Doenyas-Barak K, Efrati S
Kidney & blood pressure research 2017;42(6):1104-1118

Acute podocyte injury is not a stimulus for podocytes to migrate along the glomerular basement membrane in zebrafish larvae.

Siegerist F, Blumenthal A, Zhou W, Endlich K, Endlich N
Scientific reports 2017 Mar 2;7:43655

Glomeruli or interstitium targeted by inter-renal injections supplemented by electroporation: Still a useful tool in renal research.

Mao X, Li F, Yang N, Qi C, Zhang SQ, Zhang Z, Wu H
The journal of gene medicine 2016 Nov;18(11-12):343-352

Effects of asiaticoside on levels of podocyte cytoskeletal proteins and renal slit diaphragm proteins in adriamycin-induced rat nephropathy.

Wang Z, Liu J, Sun W
Life sciences 2013 Sep 3;93(8):352-8

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Submitted by: Proteintech Group (provider)
Main image
Experimental details: mouse colon tissue were subjected to SDS PAGE followed by western blot with 20384-1-AP(NPHS2 antibody) at dilution of 1:400
Sample type: tissue